金银花、连翘对甲型H1N1流感免疫调节通路影响的生物信息学分析

目的:基于生物信息学方法分析金银花、连翘对甲型H1N1流感免疫调节通路的影响。方法:在台湾中医药资料库,Pub Chem及Gene公众网络平台数据库查找金银花、连翘的靶蛋白及甲型H1N1流感相关基因,运用生物信息软件对检索到的数据集进行分析,解读金银花、连翘单用和连用对甲型H1N1流感相关的免疫调节生物通路的影响。结果:金银花相关靶蛋白共16个,连翘相关靶蛋白共8个,甲型H1N1流感相关基因32个;生物信息分析发现金银花和连翘有各自作用的特异性的免疫调节通路;对于巨噬细胞中白介素-12信号的生成通路和白介素-8信号通路,二者作为药对使用后,代表药物靶蛋白与生物通路相联性的-log P大于单独使用金银花或连翘;金属蛋白酶家族(MMPs)参与了多数金银花特异性作用的通路,蛋白激酶C-α(PRKCA)参与了连翘特异作用的通路。结论:对于甲型H1N1流感相关的免疫通路,金银花和连翘有各自的调节特点,作为药对使用对对巨噬细胞中白介素-12信号的生成通路和白介素-8信号通路调节更有优势,MMPs和PRKCA可能是金银花和连翘分别作用于免疫通路的关键蛋白。

Bioinformatics Analysis on Effect of Lonicerae Japonicae Flos and Forsythiae Fructus on Immune Pathway of H1N1 Influenza A

Objective: To study the effect of Lonicerae Japonicae Flos and Forsythiae Fructus on immune pathway of H1N1 Influenza A based on bioinformatics approach. Method: Target proteins of Lonicerae Japonicae Flos and Forsythiae Fructus, and genes of H1N1 Influenza A were searched in TCM Database @Taiwan, PubChem and Gene online public platform databases. Bioinformatics software was used to analyze the data sets, and explain the effect of Lonicerae Japonicae Flos and Forsythiae Fructus on immune pathway of H1N1 Influenza A. Result: There were 16 target proteins of Lonicerae Japonicae Flos, eight target proteins of Forsythiae Fructus and 32 genes of H1N1 influenza A. According to the bioinformatics analysis, Lonicerae Japonicae Flos and Forsythiae Fructus have their specific immune pathways, respectively. After the production pathway of IL-12 signaling and the pathway IL-8 signaling were treated with the drug pairs, the -log P value representing the correlation between drug target protein and biological pathway was higher than that of single administration of Lonicerae Japonicae Flos or Forsythiae Fructus.Matrix metalloproteinases(MMPs) involved in the most of specific immune pathways of Lonicerae Japonicae Flos, and PRKCA involved in the most of specific immune pathways of Forsythiae Fructus. Conclusion: Lonicerae Japonicae Flos and Forsythiae Fructus have their own characteristics in regulating specific immune pathways related to H1N1 Influenza A. When being used as a drug pair, they have more advantages in regulating the production pathway of IL-12 signaling and the pathway IL-8 signaling. MMPs and PRKCA may be the key proteins of Lonicerae Japonicae Flos and Forsythiae Fructus in regulating immune pathways.