| 冠突散囊菌不同溶剂提取物体外抗氧化活性研究 |
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| 引用本文： | 李莹,邹先伟,张小娜,王莹,唐劲天,张阳德.冠突散囊菌不同溶剂提取物体外抗氧化活性研究[J].中国实验方剂学杂志,2014,20(7):143-146. |
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| 作者姓名： | 李莹 邹先伟 张小娜 王莹 唐劲天 张阳德 |
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| 作者单位： | 北京中医药大学, 北京 100102;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;北京中医药大学, 北京 100102;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;北京中医药大学, 北京 100102;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;北京中医药大学, 北京 100102;卫生部肝胆肠外科研究中心, 长沙 410008 |
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| 基金项目： | 中国博士后科学基金项目（2013M530603） |
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| 摘 要： | 目的：研究冠突散囊菌体外抗氧化活性。方法：采用萃取法对冠突散囊菌甲醇提取物萃取获得石油醚，乙酸乙酯和正丁醇3种提取物，并以维生素C（VC）为阳性对照，用清除二苯代苦味酰基（DPPH）自由基和（ABTS）自由基测定法，对冠突散囊菌3种提取物抗氧化活性进行测定。结果：冠突散囊菌石油醚提取物清除 DPPH和ABTS+自由基半数清除浓度（IC50）分别为0.078，0.083 g·L-1 ，乙酸乙酯提取物IC50分别为0.21，0.13 g·L-1；正丁醇提取物对DPPH和ABTS+自由基几乎没有清除作用；3种提取物清除DPPH和ABTS+自由基的能力均比阳性对照VC（IC50分别为0.032，0.024 g·L-1）弱。结论：冠突散囊菌石油醚和乙酸乙酯提取物均具有抗氧化活性，且石油醚提取物活性较强。
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| 关 键 词： | 冠突散囊菌 石油醚提取物 抗氧化活性 |
| 收稿时间： | 2013/12/6 0:00:00 |
Antioxidant Activity of Different Extracts from Eurotium cristatum in vitro |
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| LI Ying,ZOU Xian-wei,ZHANG Xiao-n,WANG Ying,TANG Jin-tian and ZHANG Yang-de.Antioxidant Activity of Different Extracts from Eurotium cristatum in vitro[J].China Journal of Experimental Traditional Medical Formulae,2014,20(7):143-146. |
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| Authors: | LI Ying ZOU Xian-wei ZHANG Xiao-n WANG Ying TANG Jin-tian and ZHANG Yang-de |
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| Institution: | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100084, China;Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100084, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100084, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100084, China;Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100084, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;National Hepatobiliary Enteric Surgery Research Center, Ministry of Health, Changsha 410008, China |
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| Abstract: | Objective: To study the antioxidant activity of different extracts from Eurotium cristatum in vitro. Method: The petroleum ether, ethyl acetate and n-butanol extracts from E. cristatum were attained by the extraction method of the methanol extract of the E.cristatum. The antioxidant activity of E.cristatum was evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and (ABTS) radical scavenging assay with vitamin C(VC) as the positive control. Result: The petroleum ether extract from E. cristatum showed significant DPPH and ABTS radical scavenging activity,with 50% inhibition concentration(IC50) values of 0.078,0.083 g·L-1, respectively,the positive control VC showed IC50 values of 0.032,0.024 g·L-1, respectively.The ethyl acetate extract exhibited modest radical scavenging activity against DPPH and ABTS radicals,with IC50 values of 0.21,0.13 g·L-1, whereas the n-butanol extract did not display noticeable in vitro activity against above-mentioned radicals. Conclusion: The petroleum ether and ethyl acetate extracts from E. cristatum showed significant antioxidant activity. |
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| Keywords: | Eurotium cristatum petroleum ether extract antioxidant activity |
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