通阳宽胸颗粒对冠状动脉硬化大鼠血脂及IL-17A,VCAM-1和ICAM-1表达的影响

目的:通过观察通阳宽胸颗粒对动脉硬化大鼠的冠脉微循环障碍改善作用,探讨中西医结合治疗心血管疾病的疗效。方法:Wistar大鼠50只,随机分为通阳宽胸颗粒组(50 mg·kg~(-1)·d~(-1)),辛伐他汀组(5 mg·kg~(-1)·d~(-1)),通阳宽胸颗粒+辛伐他汀组,模型组和正常组,除正常组外,制备动脉硬化大鼠模型,正常组常规饲养,模型组和治疗组大鼠给予高脂饮食和维生素D3ip,喂饲4周后,灌胃给药8周,处死后取血清酶联免疫吸附测定(ELISA)法检测血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白(LDL-C),分析动脉斑块面积,另取主动脉行免疫组化及蛋白质免疫印迹(Western blot),观察白细胞介素-17A(IL~(-1)7A),血管间细胞黏附分子(VCAM-1)和白细胞间黏附分子(ICAM-1)的表达。结果:与正常组比较,模型组大鼠血清动脉斑块面积,TC,TG,LDL-C明显升高(P0.05);与模型组比较,治疗组血清TC,TG,LDL-C明显下降,动脉斑块面积明显缩小(P0.05,P0.01),与单独使用通阳宽胸颗粒治疗相比,中西医结合治疗组改善更明显(P0.05);与模型组比较,治疗组冠状动脉粥样硬化组织IL~(-1)7A,VCAM-1和ICAM-1的表达显著下降(P0.01),且中西医结合治疗效果优于单独使用中药。结论:通阳宽胸颗粒可通过改善血脂指标,降低动脉斑块面积,降低IL~(-1)7A,VCAM-1和ICAM-1的表达,缓解组织炎症,治疗冠状动脉粥样硬化性疾病,中西医结合治疗值得临床进一步推广。

Effect of Tongyang Kuanxiong Granule on Blood Lipid and IL-17A, VCAM-1 and ICAM-1 Expression Levels in Coronary Atherosclerosis Rats

Objective: To investigate the effects of Tongyang Kuanxiong granule on coronary microcirculation improvement in the rat with coronary arteriosclerosis, and to explore the efficacy of the combined treatment of traditional Chinese and Western medicine for cardiovascular diseases. Method: Totally 50 Wistar rats were randomly divided into normal group, model group, Tongyang Kuanxiong granule group (50 mg · kg^-1 · d^-1), Simvastatin group (5 mg · kg^-1 · d^-1), and the mix treatment group (TY+simvastin). All the rats except those in normal group were used to prepare arteriosclerosis models. The rats in normal group were given with normal diet, and the rats in model group and treatment groups were given with high-fat diet and Vitamin D₃ by ip. After 4 weeks of such feeding, the rats were treated for 8 weeks by intragastric administration. The expression levels of total cholesterol (TC), triglyceride (TG) and low density lipoprotein (LDL-C) in serum were detected by enzyme-linked immunosorbent assay (ELISA). Artery plaque area was analyzed. Aorta was taken for immunohistochemistry and Western blot to detect the expression levels of interleukin (IL)-17A, vascular cell adhesion molecule-1(VCAM)-1 and intercelluar adhesion molecule-1 (ICAM)-1. Result: As compared with the normal group, the serum artery plaque area, expression levels of TC, TG, LDL-C were significantly increased in rats of model group(P<0.05). As compared with the model group, the expression levels of TC, TG, LDL-C levels, and artery plaque area were significantly reduced in treatment groups (P<0.05, P<0.01). As compared with Tongyang Kuanxiong granule group, the improvement effect was more obvious in mixed treatment group (P<0.05). As compared with model group, the expression levels IL-17A, VCAM-1 and ICAM-1 in coronary atherosclerosis tissues were decreased in treatment groups(P<0.05), and the efficacy of mixed treatment group was better than that of Tongyang Kuanxiong granule alone group. Conclusion: Tongyang Kuanxiong granules can relieve tissue inflammation and treat coronary atherosclerotic diseases by improving blood lipid indexes, reducing the expression levels of IL-17A, VCAM-1, ICAM-1, and reducing artery plaque area, so the integrated Chinese and western medicine treatment is worthy for further promotion.