Plackett-Burman设计结合响应面法优化天麻效应成分的提取工艺

目的： 优选天麻效应成分的提取工艺。 方法： 采用回流提取法，以天麻素提取率为指标，采用Plackett-Burman设计筛选天麻效应成分的显著性影响因素，通过Box-Behnken设计对影响提取工艺的显著性因素进行优化，试验数据进行多元线性回归和二项式拟合，应用效应面法对最佳工艺进行预测分析。运用HPLC测定天麻素含量，流动相乙腈-0.05%磷酸溶液（3：97），检测波长220 nm。 结果： 加液量、提取时间和提取次数对效应成分的提取效率影响较大；二项式方程拟合度较好，预测结果较为准确，最佳工艺条件为加液量10倍，提取时间120 min，提取数3次；天麻素提取率1.392%，与预测值1.397%偏差较小。 结论： 采用Plackett-Burman设计结合响应面法优选天麻效应成分的提取工艺稳定可行，预测性较好，为天麻的资源开发提供参考。

Optimization of Extraction Process for Active Ingredients from Gastrodiae Rhizoma by Plackett-Burman Design and Response Surface Analysis

Objective: To optimize extraction process of active ingredients from Gastrodiae Rhizoma in Dachuanxiong formula. Method: Active ingredients from Gastrodiae Rhizoma was extracted by reflux,with yield of gastrodine as index,some factors affecting index were firstly evaluated by Plackett-Burman design,then extraction conditions were optimized by Box-Behnken design,experimental date were imitated by multiple regression and second-order polynomial equation,response surface analysis was adopted to predict optimum process.HPLC was employed to determine the content of gastrodine with mobile phase of acetonitrile-0.05% phosphoric acid solution(3:97) and detection wavelength at 220 nm. Result: Solvent volume,extracting times and time had significant effects on yield of active ingredients;binomial equation fitted well with good predictability,optimal extraction process was as follows:extracted thrice with solid-lipuid ratio of 1:10,120 min for each time;yield of gastrodine was 1.392%,whose deviation was small by comparing with the predicted value of 1.397%. Conclusion: Extraction process of active ingredients from Gastrodiae Rhizoma optimized by combination of Plackett-Burman design and response surface analysis was stable and feasible with good predictability,which could serve as a valuable reference for resource development of Gastrodiae Rhizoma.