桑叶多糖对长期大负荷运动训练小鼠免疫功能的影响

目的:探讨桑叶多糖对大负荷长期运动训练小鼠免疫功能的影响。方法:120只昆明种小白鼠随机分为安静对照组(C)、运动组(T)、盐水+运动组(S+T)、高剂量桑叶多糖+运动组(HM+T)、中剂量桑叶多糖+运动组(MM+T)、低剂量桑叶多糖+运动组(LM+T),每组20只。除安静对照组外,其余各组小鼠进行4周负重5%游泳训练,6次/周,50 min/次,HM+T组、MM+T组、LM+T组分别以150,200,250 mg.kg-1.d-1剂量ig,S+T组小鼠ig等体积生理盐水,连续4周。测定各组小鼠血象、体质量、巨噬细胞吞噬功能、脾脏/胸腺指数、血清凝集效价、体外抗体形成细胞的数量。结果:与S+T组比较,桑叶多糖可以明显增加小鼠白细胞(WBC)数、淋巴细胞绝对值、中性粒细胞绝对值(P<0.05～P<0.01);桑叶多糖均可不同程度增强小鼠腹腔巨噬细胞吞噬率和吞噬指数,MM+T,HM+T组吞噬率(35.7±5.17)%,(36.7±4.83)%显著高于S+T组(29.2±4.27)%,吞噬指数(3.19±1.23,2.49±1.29)显著高于S+T组(3.91±1.29)(P<0.05);桑叶多糖能增加小鼠胸腺和脾脏质量(P<0.05),提高脾脏/胸腺指数,MM+T,HM+T组脾脏指数(1.53±0.61),(1.73±0.69)mg.g-1显著高于S+T组(1.09±0.68)mg.g-1,胸腺指数(1.98±0.59),(2.21±0.63)mg.g-1显著高于S+T组(1.49±0.51)mg.g-1(P<0.05～P<0.01);桑叶多糖可以明显提高血清凝集效价以及体外抗体形成细胞的数量(P<0.05～P<0.01)。结论:桑叶多糖能增强长期大负荷运动小鼠机体免疫功能,调节免疫细胞以及免疫分子。其中桑叶多糖中、高剂量的效果明显。

Effect of Mulberry Leaves Polysaccharide on Immune Function in Mice with Long-term Heavy Training

Objective:To explore the effect of mulberry leaves polysaccharide on the immune function of mice with long-term heavy training. Method: One hundred and twenty mice were divided into six groups at random: quiet control group, training group, saline movement group, high dosage mulberry leaves polysaccharide movement group, medium dosage mulberry leaves polysaccharide movement group(250,200,150 mg·kg^-1·d^-1), low dosage mulberry leaves polysaccharide movement group, with 20 mice in every group. In addition to quiet control group, other groups of mice do swimming training with 5% weight for 50 minutes, with 6 times a week. The mice in mulberry leaves polysaccharide movement group were orally given with the dosage of 150,200, 250 mg·kg^-1·d^-1 while the mice in saline movement group were given with normal saline for 4 weeks.The hemogram, body weight, phagocytic function of macrophages, index of spleen and thymus. serum agglutination titer, the quantity of plaque-forming cell were tested. Result: Compared with the saline movement group,there was a significant increase in WBC, Lym absolute value, absolute value of neutrophilic granulocyte, mulberry leaves polysaccharid could increase phagocytic percentage and phagocytic index of mice peritoneal macrophage,which was significantly higher in phagocytic percentage of mulberry leaves polysaccharid medium high dose group(35.7±5.17)%,(36.7±4.83)% than that in the saline movement group (29.2±4.27)% and was significantly higher in phagocytic index of mulberry leaves polysaccharid medium high dose group(3.19±1.23,2.49±1.29) than that in the saline movement group(3.91±1.29)(P<0.05). polysaccharid could increase the weight of thymus and spleen significantly(P<0.05),and could increase index of spleen and thymus. Mulberry Leaves polysaccharid could increase serum agglutination titer and the quantity of plaque-forming cell significantly(P<0.05-P<0.01). Concllusion: Mulberry leaves polysaccharides can enhance long-term heavy load exercise mice immune function, regulation of immune cells and immune molecules. The effect in medium and high dose mulberry leaves polysaccharides is more significant.