黄芩、黄连配伍前后的主要化学成分含量变化

目的:考察相须药对的配伍对主要活性成分煎出量的影响。方法:选择黄芩-黄连药对为研究对象,以黄芩苷和盐酸小檗碱含量为指标,采用HPLC测定含量,选取加水量、提取次数、提取时间及黄芩-黄连比为考察因素,通过混合均匀设计试验优选煎煮工艺。考察不同比例配伍的黄芩-黄连共煎液与单煎混合液化学成分的异同,并采用三维效应面及三角能级图的形式将两指标成分进行直观对比,重点关注因两味中药在共煎而产生的化学成分动态变化现象。结果:黄连主要活性成分的煎出量与两者的配伍比例显著性相关;而黄芩活性成分的煎出量与煎煮时影响因素有关。黄连用量为黄芩用量2,3,5倍时溶液中两者含量相对较高,其他比例时均较低。结论:可通过研究药对的不同配比与其各自化学成分动态变化关系,揭示其临床使用疗效。

Content Change of Main Chemical Composition Before and After Compatibility of Scutellaria baicalensis and Coptis chinensis

Objective:To study effect of compatibility of traditional Chinese medicine pair on decocting rate of main active ingredient. Method: With pair of Scutellaria baicalensis and Coptis chinensis as study object,the content of baicalin and berberine hydrochloride as indexes,which were determined by HPLC,the amount of water,extraction times,extraction time and ratio of S. baicalensis and C. chinensis were chosen as factors,boiling technology was optimized by mixing uniformity design test.Difference of chemical composition between total decoction(with different proportion of S. baicalensis and C. chinensis) and mixed liquid of single decoction of them was investigated,and two target ingredients were visual contrasted by three dimensional response surface and triangle energy level diagram,dynamic change phenomenon of chemical composition was focused on,which due to mixed decoction of S. baicalensis and C. chinensis. Result: The decocting content of main active ingredient from C. chinensis showed significantly correlation with compatibility proportion of S. baicalensis and C. chinensis;But the decocting content of active ingredient from S. baicalensis was concerned with decoction influencing factors.The contents of two index component were lower with other proportions of S. baicalensis and C. chinensis,except the amount of C. chinensis was 2,3,5 times for S. baicalensis. Conclusion: Clinical efficacy of pair-Chinese materia medica could be revealed by study on dynamical change relationship of different compatibility proportions and their chemical composition.