人参拮抗高皮质激素血症致神经元损伤的作用

目的:探讨人参保护高皮质激素血症致神经元损伤的作用机制。方法:皮下注射皮质酮建立高皮质激素血症的动物模型,在模型的制备过程中同时ig给予高、中、低(7.2,3.6,1.8 g.kg-1)3个剂量的人参水煎剂,实验进行4周,行为学检测后处死动物,取脑剥离海马,对神经微丝蛋白(NF-L)、突触囊泡总蛋白(SYP)、脑源性神经营养因子(BDNF)、胶质源性神经营养因子(GDNF)进行蛋白免疫印迹。结果:模型组动物在行为学检测中,静止不动时间明显增加,与正常组有显著差别;体重较正常组显著降低并有显著差异。相关蛋白检测NF-L,SYP,BDNF,GDNF表达水平与正常组比较显著下调。人参高、中剂量组能够改善动物行为学变化及体重变化,人参各剂量组对神经元蛋白及神经营养因子均表现出明显的上调作用。结论:人参可通过上调神经营养因子的表达发挥神经元保护作用。

Preventive Effects of Panax ginseng on Neuron Damage Induced by Hypercortisolism

Objective: To explore the mechanism underlying preventive effects of ginseng on neuron damage induced by hypercortisolism. Method: Mouse model of hypercortisolism was developed by subcutaneous corticosterone injection.Different dosage of ginseng water decoction (7.2,3.6,1.8 g ·kg^-1) was orally administrated to mice during reproducing animal model.After four weeks,the mice were sacrificed by decapitation after behavioral tests.Then,the hippocampal tissues were rapidly collected for Western blot analysis of four important proteins,neurofilament light chain protein (NF-L),synaptic vescle protein (SYP),brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF). Result: In contrast to the control,depression-like behavior was obviously increased,and protein levels of NF-L,SYP,BDNF and GDNF were significantly decreased in the model group.In contrast to the model group,all dosage of ginseng water decoction not only could significantly improve depression-like behavior,but also obviously up-regulate protein levels of NF-L,SYP,BDNF and GDNF in the hippocampal tissues. Conclution: Ginseng can provide neuroprotection by up-regulating protein expression of neurotrophic factors.