菟丝子总黄酮对过氧化氢损伤的血管内皮细胞的保护作用

目的:探讨菟丝子总黄酮对H202损伤的人脐静脉内皮细胞(HUVECs)的保护作用.方法:于37℃、5％ CO2、95％空气饱和湿度的培养箱内体外培养内皮细胞,将细胞分为4组,即正常对照组:加入DMEM培养液(pH 7.2 ～7.4)、菟丝子对照组(菟丝子0.5mg·L-1)、模型组(过氧化氢终浓度为200 mmol·L-)、菟丝子+过氧化氢组(菟丝子0.5mg· L-1,过氧化氢200 mmol·L-),研究菟丝子总黄酮对受损血管内皮细胞内超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、乳酸脱氢酶(LDH)释放情况、谷胱甘肽过氧化物酶(GSH-Px)活性及对细胞形态学的影响.结果:菟丝子总黄酮(0.5 mg·L-1)能明显减轻H2O2对HUVECs所致的氧化损伤,可明显改善氧化损伤的HUVECs形态学变化,H2O2模型组(200 mmol·L-)与空白组比较,LDH释放和MDA含量显著升高(P＜0.01,P＜0.05),SOD释放水平和GSH-Px活力显著下降(P＜0.01),菟丝子(0.5mg·L-1)+ H2O2(200 mmol·L-1)组与H2O2模型组(200 mmol·L-1)比较,LDH释放和MDA含量显著下降(P＜0.01,P＜0.05),SOD释放水平和GSH-Px活力显著升高(P＜0.01).结论:菟丝子总黄酮对H202诱导的HUVECs的损伤具有保护作用.

Protective Effects of Flavonoids Extracted from Cuscutae Semen on Human Umbilical Vein Endothelial Cells Injured by H2O2

Objective: To study the protective effects of flavonoids extracted from Cuscutae Semen (FCS) on human umbilical vein endothelial cells injured by H₂O₂. Method: Vein endothelial cells were cultured in vitro and divided into four groups which was normal control,FCS control,model,FSC+H₂O₂respectively. We tested the influence of FCS on the endothelial cells oxidative damage induced by H₂O₂ the indexes of superoxide dismutase(SOD),malanyldiadehyde(MDA),lactate dehydrogenase(LDH),glutathione peroxidase(GSH-Px)and apoptosis were measured. Result: FCS could remarkable lessen oxidative damage of endothelial cell induced by H₂O₂, compared with normal control groups,the levels of LDH and MDA in model group remarkably increased and the activity of SOD and GSH-Px obviously decreased;while the groups pretreated by FCS,compared with model group,the levels of LDH and MDA obviously decreased and the activity of SOD and GSH-Px increased. Conclusion: FCS has protective effects on human umbilical vein endothelial cells injured by H₂O₂.