巴豆中巴豆苷的提取纯化及制备

目的:建立一种从巴豆中提取纯化及制备巴豆苷的方法.方法:以巴豆苷得率为指标,采用正交试验设计优化巴豆苷的提取工艺参数；考察大孔树吸附脂纯化工艺条件；应用制备型液相色谱分离高纯度巴豆苷.结果:最佳巴豆苷提取工艺参数为8倍量20％乙醇提取3次,每次40 min;大孔树脂吸附初步纯化后,制备液相分离出巴豆苷单体,质量分数达98％,可作为对照品.结论:该方法简便易行,可用于分离制备高纯度巴豆苷样品.

Extraction Purification and Preparation of Isoguanosine from Croton tiglium

Objective: To establish a method for extracting, purifying and preparing isoguanosine from Croton tiglium. Method: Orthogonal design was used to optimize extraction technology parameters with the content of isoguanosine as index; Studying purification process of isoguanosine with macroporous resin and preparing high purity isoguanosine by preparative liquid chromatography. Result: Optimum extraction technology of isoguanosine was extracted 3 times with 8 times the amount of 20% ethanol, 40 min each time. After preliminarily purifying by macroporous resin,isoguanosine was isolated from purified extracting solution and the purity was up to 98%. Conclusion: This method was simple and easy to operate, also could be used for preprartion high purity isoguanosine samples.