侗药五味止泻汤对溃疡性结肠炎大鼠血清IL-1,IL-10 的影响

目的:研究侗药五味止泻汤对溃疡性结肠炎(UC)大鼠结肠损伤的修复作用及对血清IL-1和IL-10的影响.方法:以2,4,6-三硝基苯磺酸和乙醇灌肠法制备UC大鼠模型,将60只SD大鼠随机分为正常组,模型组,侗药五味止泻汤低、中、高剂量组(5.5,11,22 g·kg-1)和美沙拉嗪(0.5 g·kg-1)组,共6组,每组10只.低、中、高剂量组分别给予不同剂量侗药五味止泻汤ig,美沙拉嗪组予以美沙拉嗪ig,正常组及模型组灌服等体积蒸馏水.10d后观察大鼠一般情况及组织病理学改变,透射电镜观察结肠超微结构改变,采用ELISA法检测各组大鼠的血清白介素-1(IL-1)、白介素-10(IL-10)的水平.结果:与模型组比较,侗药五味止泻汤3个剂量组均能有效提高大鼠血清IL-10含量,降低IL-1含量(P＜0.01),并对损伤的结肠黏膜有明显的修复作用,降低结肠黏膜损伤指数(P＜0.01).结论:侗药五味止泻汤通过抑制促炎细胞因子IL-1,促进抗炎细胞因子IL-10生成,发挥对UC的治疗作用.

Effect of Dongyao Wuwei Zhixie Decoction on Serum Levels of IL-1 and IL-10 in Rats with Ulcerative Colitis

Objective: To study the effect of Dongyao Wuwei Zhixie decoction on serum levels of interleukin-1(IL-1)and interleukin-10(IL-10) in rats with intestinal ulcerative colitis and. Method: Ulcerative colitis rat model was copied by 2,4,6-TNBS. Sixty SD rats were randomly divided into normal group,model group,Dongyao Wuwei Zhixie decoction low,middle and high dose group(5.5,11,22 g·kg^-1),Mesalazie group (0.5 g·kg^-1),10 in each group. The general situation and histopathological changes were observed.The ultrastructural changes of UC model rats were investigated by transmission electron microscope and the rat serum levels of IL-1,IL-10 were detected by ELISA 10 days later. Result: Dongyao Wuwei Zhixie decoction effectively improved the serum levels of IL-10, reduced the serum levels of IL-1, and had significant effect of repairing the intestinal mucosal injury. Conclusion: Dongyao Wuwei Zhixie decoction achieves a therapeutic effect by inhibiting the production of proinflammatory cytokines IL-1 and improving the production of antiinflammatory cytokines IL-10.