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当归、川芎配伍闪式提取工艺优选
引用本文:王玥,杜守颖,戴俊东,陆洋,魏长玲,陆显进,黎迎,李慧云,赵静宜.当归、川芎配伍闪式提取工艺优选[J].中国实验方剂学杂志,2012,18(17):5-8.
作者姓名:王玥  杜守颖  戴俊东  陆洋  魏长玲  陆显进  黎迎  李慧云  赵静宜
作者单位:北京中医药大学,北京,100102
基金项目:国家自然科学基金面上项目(81073057);教育部博士点基金项目(20090013110007);北京中医药大学复方中药制药研究创新团队(2011-CXTD-13);国家重点实验室开放基金项目(SKL2010Z0304);国家重点实验室开放基金项目(SKL2010M0302)
摘    要:目的:优选当归、川芎配伍提取工艺,比较川芎、当归单提和共提液中阿魏酸的含量变化.方法:以阿魏酸为指标,HPLC测定阿魏酸含量,选取提取时间、提取电压、乙醇用量及乙醇体积分数为考察因素,采用正交试验设计优选当归-川芎配伍闪式提取工艺,并用该优选工艺分别提取当归和川芎,比较单提、共提液中阿魏酸含量变化,探讨当归-川芎配伍的增效机制.结果:最佳提取工艺为20倍量65%乙醇于100 V电压下闪式提取1.5 min;当归-川芎共提液中阿魏酸的含量与单提液之和相近.结论:该优选工艺简单、经济,证实了当归、川芎配伍药理作用增强的原因并非阿魏酸含量的提高引起.

关 键 词:闪式提取  当归  川芎  阿魏酸  正交试验
收稿时间:2012/2/28 0:00:00

Optimization of Flash Type Extraction Technology for Ligusticum chunxiong and Angelica sinensis
WANG Yue,DU Shou-ying,DAI Jun-dong,LU Yang,WEI Chang-ling,LU Xian-jin,LI Ying,LI Hui-yun and ZHAO Jing-yi.Optimization of Flash Type Extraction Technology for Ligusticum chunxiong and Angelica sinensis [J].China Journal of Experimental Traditional Medical Formulae,2012,18(17):5-8.
Authors:WANG Yue  DU Shou-ying  DAI Jun-dong  LU Yang  WEI Chang-ling  LU Xian-jin  LI Ying  LI Hui-yun and ZHAO Jing-yi
Institution:Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100102,China
Abstract:Objective: To optimize compatibility extraction technology of Ligusticum chunxiong and Angelica sinensis,and compare the content of ferulic acid in Chinese herbal compound contain L. chunxiong and A. sinensis pair compatibility and single one. Method: With the content of ferulic acid as index which was determined by HPLC, compatibility extraction technology of L. chunxiong and A. sinensis was optimized by orthogonal test design with extraction time,extraction voltage,ethanol consumption and ethanol volume fraction as factors,and optimized process was used to extract L. chunxiong and A. sinensis single,the content variation of ferulic acid were compared in compatibility and single extraction,in order to investigate synergism mechanism of compatibility of L. chunxiong and A. sinensis. Result: Optimum extraction technology was:extracted 1.5 min with 20 times the amount of 65% ethanol,extraction voltage 100 V;The content of ferulic acid in compatibility extraction liquid was similar with the total content in single extraction liquid. Conclusion: This optimized technology was simple and economic,it confirmed that reason for enhancing pharmacological effect of compatibility with L. chunxiong and A. sinensis wasn't because of increasing the content of ferulic acid.
Keywords:flash type extraction  Angelica sinensis  Ligusticum chunxiong  ferulic acid  orthogonal test
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