17-甲氧基-7-羟基-苯并呋喃查尔酮对大鼠凝血功能和血小板聚集的影响

目的:研究17-甲氧基-7-羟基-苯并呋喃查尔酮(YLSC)对体内外血小板聚集和凝血功能的影响.方法:将50只SD大鼠随机分为5组:对照组(含0.5％ DMSO的生理盐水),YLSC低、中、高剂量组(2.5,5,10 mg·kg-1),阳性药阿司匹林组(10 mg·kg-1).尾静脉注射相应药物1周后,腹主动脉采血,分别加入二磷酸腺苷(ADP)、胶原和花生四烯酸(AA)诱导血小板聚集,测定大鼠体内外血小板聚集率；同时观察YLSC对大鼠凝血酶原时间(PT)、活化部分凝血激酶时间(APTT)及凝血酶时间(TT)的影响.结果:与对照组相比,YLSC能显著抑制ADP和胶原诱导的大鼠体内、外血小板最大聚集率(P＜0.05或P＜0.01),最大抑制率分别为38.4％,42.5％,对AA诱导的血小板聚集无明显抑制作用；YLSC能显著延长大鼠血浆TT,APTT(与对照组比较,P＜0.05或P＜0.01),对PT无显著影响.结论:YLSC具有对抗血小板聚集和抗凝血作用.

Effect of 17-methoxyl-7-hydroxyl-benzofuran Chalcone on Blood Coagulation and Platelet Aggregation

Objective:To investigate the inhibitory effects of 17-methoxyl-7-hydroxyl-benzofuran chalcone(YLSC) on platelet aggregation and coagulation function in rats. Method: Fifty SD rats were randomly divided into five groups:control group, aspirin group(10 mg·kg^-1) and YLSC groups(2.5, 5,10 mg·kg^-1). YLSC and aspirin were administrated through sublingual vein for one week.Inhibition rates of platelet aggregation by YLSC in vivo and vitro were determined in the model of platelet aggregation induced by adenosine diphosphate (ADP), collagen and arachidonic acid(AA).The anticoagulant effect of YLSC was evaluated by using thrombin time(TT), prothrombin time(PT), and activated partial thromboplastin time(APTT) assays. Result: Compared with the control group, YLSC could significantly inhibit platelet aggregation induced by ADP and collagen(P<0.05 or P<0.01), but did not inhibit platelet aggregation induced by AA. YLSC significantly prolonged the TT and APTT in a dose-dependent manner(P<0.05 or P<0.01). Wereas, YLSC did not show significant prolonging effect in PT assays. Conclusion: YLSC can exert remarkable effects against platelet aggregation and coagulation.