多指标评价米泔水漂苍术对湿盛困脾脾虚大鼠的药效作用

目的:研究樟帮特色米泔水漂苍术与其麸炒品、生品对湿盛困脾大鼠的疗效,并考察其燥性,以发掘特色饮片、提高饮片质量,为苍术的临床用药提供科学数据。方法:采用外湿困脾、饮食不节、劳倦过度的复合方式复制湿盛困脾脾虚大鼠模型,将SD大鼠随机分成5组,每组8只,分别设空白组、模型组、米泔水漂苍术组、麸炒苍术组、苍术生品组,每天记录体重、饮水量、尿量,治疗12 d后采用炭末灌胃法测量胃排空速率和肠推进率,测量脾指数,检测血清淀粉酶(AMS),D-木糖及胃泌素(GAS)的含量。结果:与模型组相比,各给药治疗组体重均有恢复趋势;体液消耗量各给药组均高于模型组,且苍术生品组麸炒苍术组米泔水漂苍术组;脾指数各给药组显著高于模型组,其中米泔水漂苍术组与麸炒苍术组显著高于空白组;各给药组的AMS,D-木糖,GAS水平较模型组均显著提高,其中米泔水漂苍术组与麸炒苍术组显著高于空白组。结论:米泔水漂苍术与其麸炒品、生品都对湿盛困脾脾虚大鼠有一定药效作用,且麸炒品和米泔水制品显著优于生品,且均具有良好的健脾和胃、调节胃肠道功能与分泌的作用。燥性排序为米泔水漂制品麸炒品生品。

Evaluation of Effect of Rice-washed Water Bleaching Atractylodis Rhizoma on Spleen Deficiency Rats with Dampness Accumulation in Spleen by Multiple Indexes

Objective: To study on the effect of rice-washed water bleaching products,fried products with bran and raw products of Atractylodis Rhizoma on spleen deficiency rats,and investigate their dryness nature,so as to provide scientific data for clinical medication of Atractylodis Rhizoma. Method: Model of spleen deficiency in rats was prepared by complex method of exogenous dampness disturbing spleen,improper diet and overstrain.SD rats were randomly divided into five groups,8 rats in each group,including the blank group,model group,rice-washed water bleaching Atractylodis Rhizoma group,Atractylodis Rhizoma fried with bran group,crude Atractylodis Rhizoma group.Notes about daily weight,water intake and urine volume were recorded daily.After treatment with 12 days,the gastric emptying velocity and intestinal propulsive ratio were measured by charcoal gavage and spleen index was measured,contents of serum amylase(AMS),D-xylose and gastrin(GAS) were determined. Result: Compared with the model group,each drug treatment group showed weight recovery trend.Fluid consumption in each drug group was higher than that in the model group,and the order was crude Atractylodis Rhizoma group >Atractylodis Rhizoma fried with bran group >rice-washed water bleaching Atractylodis Rhizoma group.The spleen index in each drug group was significantly higher than that in the model group,and Atractylodis Rhizoma fried with bran group and rice-washed water bleaching Atractylodis Rhizoma group were significantly higher than the blank group.Levels of AMS,D-xylose and GAS in each drug group were significantly higher than those in the model group,and Atractylodis Rhizoma fried with bran group and rice-washed water bleaching Atractylodis Rhizoma group were significantly higher than the blank group. Conclusion: Processed products of Atractylodis Rhizoma all have a certain effect on spleen deficiency rats,effects of rice-washed water bleaching products and fried products with bran are significantly better than that of raw products,they all have a good effect of strengthening the spleen and stomach,regulating gastrointestinal function and secretion.Dryness nature is in the order of rice-washed water bleaching products< fried products with bran< raw products.