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超临界CO2 萃取法与水蒸气蒸馏法提取桂枝-干姜 药对的化学成分比较
引用本文:黄鹤归,叶晓川,田连起,李娜,邹甜,周爱军,刘焱文.超临界CO2 萃取法与水蒸气蒸馏法提取桂枝-干姜 药对的化学成分比较[J].中国实验方剂学杂志,2011,17(24):85-92.
作者姓名:黄鹤归  叶晓川  田连起  李娜  邹甜  周爱军  刘焱文
作者单位:1. 湖北中医药大学中药资源与中药复方省部共建教育部重点实验室,武汉,430061
2. 湖北中医药大学中药资源与中药复方省部共建教育部重点实验室,武汉430061;河南中医学院,郑州 450008
3. 东莞市沙田医院,广东东莞,523981
基金项目:东莞市科技局项目(东科[2010]111号)
摘    要:目的:探讨超临界CO2萃取法和水蒸气蒸馏法提取桂枝-干姜药对有效成分的差异,阐释该药配伍前后有效成分的变化情况,以期揭示其药效物质基础。方法:通过超临界CO2萃取法与水蒸气蒸法提取桂枝、干姜、桂枝-干姜药对化学成分,通过GC-MS分析鉴定,比对该药对配伍前后成分变化,总结其变化规律。结果:超临界CO2萃取桂枝、干姜、桂枝-干姜药对萃取物的得率分别为0.69%,3.66%,1.82%,分别从中鉴定出44,57,65个成分,其占萃取物含量分别为84.22%,91.25%,90.13%;水蒸气蒸馏提取桂枝、干姜、桂枝-干姜药对挥发油的得率分别为0.25%,1.75%,1.05%,分别从中鉴定出19,50,57个成分,其占挥发油含量分别为85.35%,93.47%,93.11%。结论:桂枝-干姜配伍有促进成分溶出作用,两者配伍不是成分的简单叠加;超临界CO2萃取法的提取效率明显高于传统水蒸气蒸馏法。

关 键 词:桂枝  干姜  气相色谱-质谱法  水蒸气蒸馏法  超临界流体萃取法
收稿时间:2011/8/12 0:00:00

2011-10-17 9:40 http://www.cnki.net/kcms/detail/11.3495.R.20111017.0940.005.html Comparative Study on Supercritical Fluid Extraction and Steam Distillation in Extracting Chemical Constituents and Relative Contents of Volatile Oil from Compatibility of Ramulus Cinnamomi and Rhizoma Zingiberis
HUANG He-gui,YE Xiao-chuan,TIAN Lian-qi,LI N,ZOU Tian,ZHOU Ai-jun and LIU Yan-wen.2011-10-17 9:40 http://www.cnki.net/kcms/detail/11.3495.R.20111017.0940.005.html Comparative Study on Supercritical Fluid Extraction and Steam Distillation in Extracting Chemical Constituents and Relative Contents of Volatile Oil from Compatibility of Ramulus Cinnamomi and Rhizoma Zingiberis[J].China Journal of Experimental Traditional Medical Formulae,2011,17(24):85-92.
Authors:HUANG He-gui  YE Xiao-chuan  TIAN Lian-qi  LI N  ZOU Tian  ZHOU Ai-jun and LIU Yan-wen
Institution:Provincial and Ministry of Education Key Laboratory of Resoure Science and Chinese Herbal Compound in Chinese Medicine Hubei University of Traditional Chinese Medicint(TCM), Wuhan 430061, China;Provincial and Ministry of Education Key Laboratory of Resoure Science and Chinese Herbal Compound in Chinese Medicine Hubei University of Traditional Chinese Medicint(TCM), Wuhan 430061, China;Provincial and Ministry of Education Key Laboratory of Resoure Science and Chinese Herbal Compound in Chinese Medicine Hubei University of Traditional Chinese Medicint(TCM), Wuhan 430061, China; Henan University of TCM, Zhengzhou 450008, China;Provincial and Ministry of Education Key Laboratory of Resoure Science and Chinese Herbal Compound in Chinese Medicine Hubei University of Traditional Chinese Medicint(TCM), Wuhan 430061, China;Provincial and Ministry of Education Key Laboratory of Resoure Science and Chinese Herbal Compound in Chinese Medicine Hubei University of Traditional Chinese Medicint(TCM), Wuhan 430061, China;Shatian Hospital of Dongguan City, Dongguan 523981, China;Provincial and Ministry of Education Key Laboratory of Resoure Science and Chinese Herbal Compound in Chinese Medicine Hubei University of Traditional Chinese Medicint(TCM), Wuhan 430061, China
Abstract:Objective :To investigate the changes of chemical compositions before and after the compatibility of Ramulus Cinnamomi and Rhizoma Zingiberis in order to explore the effective material base through.comparative study on supercritical fluid extraction and steam distillation in extracting chemical constituents and relative contents of the volatile oil from the compatibility of Ramulus Cinnamomi and Rhizoma Zingiberis. Method: Using supercritical fluid extraction and steam distillation, chemicals in Ramulus Cinnamomi, Rhizoma Zingiberis and herbal pair Ramulus Cinnamomi-Rhizoma Zingiberis were extracted. The chemicals were indenified before and after mixing the medicines by using GC-MS. The changes in the mixture were summerized and recorded. Result: The mass percentage of extractions from Ramulus Cinnamomi, Rhizoma Zingiberis, and the herbal pair by SFE-CO2 were 0.69%, 3.66%, 1.82%, respectively, and 44, 57, and 65 chemical components were identified from the three extractions. The identified components were 84.22%, 91.25%, and 90.13% of the extractions, respectively. Though steam distillation, the mass percentage of collected volatile chemicals were 0.25%, 1.75%, 1.05% from Ramulus Cinnamomi, Rhizoma Zingiberis, and the herbal pair. 19, 50, 57 chemical components were identified. The identified components were 85.35%, 93.47%, and 93.11% of the extractions, respectively. Conclusion : The compatibility of Ramulus Cinnamomi and Rhizoma Zingiberis can promote dissolution of the chemicals in the medicines, and the compatibility is not in simple mixture of the components, and extraction efficiency by SFE-CO2 is higher than that by steam distillation.
Keywords:Ramulus Cinnamomi(RC)  Rhizoma Zingiberis(RZ)  GC-MS  steam distillation  supercritic fluid extraction
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