| 紫薇不同部位药材质量控制方法 |
| |
| 引用本文： | 刘春花,潘洁,孙佳,陆苑,马雪,李勇军,龙庆德,张旭,王爱民,王永林.紫薇不同部位药材质量控制方法[J].中国实验方剂学杂志,2018,24(9):64-69. |
| |
| 作者姓名： | 刘春花 潘洁 孙佳 陆苑 马雪 李勇军 龙庆德 张旭 王爱民 王永林 |
| |
| 作者单位： | 贵州医科大学 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004;贵州医科大学 药学院, 贵阳 550004,贵州医科大学 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004,贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004;贵州医科大学 贵州省药物制剂重点实验室, 贵阳 550004,贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004;贵州医科大学 贵州省药物制剂重点实验室, 贵阳 550004,贵州医科大学 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004,贵州医科大学 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004,贵州医科大学 药学院, 贵阳 550004,贵州医科大学 药学院, 贵阳 550004,贵州医科大学 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004,贵州医科大学 药物植物功效与利用国家重点实验室, 贵阳 550004;贵州医科大学 贵州省药物制剂重点实验室, 贵阳 550004 |
| |
| 基金项目： | 贵州省民族药药效物质基础研究科技创新人才团队项目（黔科合平台人才[2016]5613）；贵州省高层次创新型人才培养（百层次）（黔科合平台人才[2016]5677）；贵州省中药材、民族药材质量标准研究项目（105，103，104） |
| |
| 摘 要： | 目的:建立紫薇不同部位药材(花、叶、皮)薄层色谱鉴别方法,并建立紫薇不同部位药材中鞣花酸含量含量测定方法,测定不同批次紫薇不同部位药材中水分、灰分和浸出物,对紫薇不同部位药材进行有效的质量控制。方法:采用薄层色谱对紫薇不同部位药材进行鉴别,展开系统为丙酮-石油醚-甲酸(3∶5∶0.2),分别于365,254 nm紫外光或日光下检视;建立HPLC-DAD测定紫薇不同部位药材中鞣花酸的含量,UltimateAQ-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.2%磷酸水(15∶85),流速1 mL·min~(-1),检测波长254 nm,柱温40℃;进样量5~10μL;依据2015年版《中国药典》四部通则测定了紫薇不同部位药材中的醇溶性浸出物、灰分和水分。结果:紫薇不同部位药材的薄层色谱鉴别主斑点分离较好。鞣花酸在0.276 8~55.36 mg·L~(-1)呈良好的线性关系(r=0.999 8),紫薇花、叶和皮中鞣花酸的平均加样回收率分别为99.64%,99.73%,99.40%,不同批次和产地的紫薇样品中鞣花酸含量存在一定差异,紫薇花中鞣花酸含量较叶和皮高。紫薇花、叶、皮中醇溶性浸出物平均质量分数分别为18.61%,18.67%,3.78%,总灰分平均质量分数分别为6.50%,7.47%,5.62%,水分平均质量分数分别为10.51%,11.41%和14.22%。结论:该方法简便、准确、可靠,可以为更有效地控制紫薇药材质量提供依据。
|
| 关 键 词： | 紫薇 花 叶 皮 鞣花酸 清热解毒 |
| 收稿时间： | 2017/8/3 0:00:00 |
Quality Control Method for Different Parts of Lagerstroemia indica |
| |
| LIU Chun-hu,PAN Jie,SUN Ji,LU Yuan,MA Xue,LI Yong-jun,LONG Qing-de,ZHANG Xu,WANG Ai-min and WANG Yong-lin.Quality Control Method for Different Parts of Lagerstroemia indica[J].China Journal of Experimental Traditional Medical Formulae,2018,24(9):64-69. |
| |
| Authors: | LIU Chun-hu PAN Jie SUN Ji LU Yuan MA Xue LI Yong-jun LONG Qing-de ZHANG Xu WANG Ai-min and WANG Yong-lin |
| |
| Institution: | Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine(Ministry of Education), Guizhou Medical University, Guiyang 550004, China;State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China;School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine(Ministry of Education), Guizhou Medical University, Guiyang 550004, China;State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China,State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China;Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China,State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China;Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine(Ministry of Education), Guizhou Medical University, Guiyang 550004, China;State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine(Ministry of Education), Guizhou Medical University, Guiyang 550004, China;State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China,School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine(Ministry of Education), Guizhou Medical University, Guiyang 550004, China;State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China and State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China;Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China |
| |
| Abstract: | |
| |
| Keywords: | Lagerstroemia indica flower leave bark ellagic acid clearing heat and detoxicating |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
|
