丹参注射液对老龄大鼠红细胞膜组分的影响

目的:从血液流变学和红细胞膜组分的变化探讨丹参注射液改善老龄大鼠血瘀证的作用机制.方法:70 ～72周龄Wistar大鼠30只,雌雄各半,随机分为丹参注射液(按生药量计)高剂量组(4.4 g·kg-1)、低剂量组(1.1 g·kg-1)和模型组.另外10只青龄大鼠作为青龄对照组,ip给药14 d后,取血测定全血黏度、血浆黏度、红细胞变形指数以及红细胞膜各组分等指标.结果:与青龄对照组比较,模型组全血黏度显著升高(P＜0.05),血浆黏度升高极显著(P＜0.01)；在200 s-1,500 s-1切变率下红细胞变形指数均降低极显著(P＜0.01),1000s-1切变率下红细胞变形指数显著降低(P＜0.05).与模型组相比丹参注射液组能够显著降低老龄大鼠的全血黏度(P＜0.01)和血浆黏度(P＜0.05),明显提高红细胞变形能力(P＜0.01)；并且能显著增加老龄大鼠红细胞膜唾液酸含量和巯基含量(P＜0.05),显著提高红细胞膜Na+-K+-ATP酶和SOD(超氧化物歧化酶)活性(P＜0.01,P＜0.01),降低MDA(丙二醛)水平(P＜0.01).结论:丹参注射液可能是通过增加红细胞膜表面唾液酸含量、影响膜上脂质代谢、提高机体抗氧化能力,以及改变膜上负电荷荷电量等方面来改善老龄大鼠的血液流变学特性.

Effect of Danshen Injection on the Components of Erythrocyte Membrane in the Aged Rats

Objective: To investigate the mechanism of action in blood stasis syndrome of aged rats by Danshen injection. And the change of hemorheology and the components of erythrocyte membrane were studied. Method: Thirty Wistar rats which were 70-72 weeks were randomly assigned to Danshen injection high-dose group (drug 4.4 g·kg^-1), low-dose group (drug 1.1 g·kg^-1) and model group with 10 rats in each group. Another ten younger Wistar rats were as young control group. The related indexes of hemorheology such as the whole blood viscosity, plasma viscosity, the red blood cell deformability and the components of erythrocyte membrane were determined after giving medicine 14 days by intraperitoneal injection. Result: Compared with the control group, the whole blood viscosity and plasma viscosity of the model group were increased (P<0.05, P<0.01). And the red blood cell deformability was decreased significantly under 200 s^-1 and 500 s^-1 (P<0.01) and 1 000 s^-1 (P<0.01). Compared with the model group, the Danshen injection group was effective. The whole blood viscosity (P<0.01), plasma viscosity (P<0.05), the content of malondialdehyde (MDA) in erythrocyte membrane (P<0.01)were decreased. The red blood cell deformability (P<0.01) and the content of sialic acid, sulfhydryl, superoxide dismutase (SOD) and Na⁺-K⁺-ATPase activity (P<0.05) in erythrocyte membrane were increased. Conclusion: Danshen injection can improve the situation of blood stasis through increasing the content of sialic acid and SOD and reducing the content of MDA in erythrocyte membrane. It indicated that the metabolism of lipid was affected, ability of anti-oxidation was enhanced, the content of oxygen radical was decreased and the carrying capacity of negative charge in erythrocyte membrane was improved.