小半夏汤对豚鼠离体肠管运动的影响

目的: 观察小半夏汤对豚鼠离体肠管收缩的影响,初步探讨其止吐的作用机制。 方法: 利用离体浴槽,观察小半夏汤(生药,下同) 0, 0.1, 0.25, 0.5, 1, 2.5, 5, 10, 20, 40 g·L^-1]对豚鼠离体肠管自主收缩平均张力、振幅和频率的影响;以呕吐相关受体激动剂乙酰胆碱(Ach)、组胺(His)、五羟色胺(5-HT)、多巴胺(DA)为工具药,观察小半夏汤0, 2, 5, 10 g·L^-1对各工具药致豚鼠离体肠管收缩平均张力、振幅、频率和最大张力的影响。 结果: 小半夏汤能剂量依赖性地抑制豚鼠离体肠管自主收缩,降低其收缩平均张力、振幅和频率(与空白对照组相比,P<0.05或P<0.01);先加Ach,His,5-HT再加小半夏汤2,5,10 g·L^-1或小半夏汤2,5,10 g·L^-1预处理均能明显抑制3种工具药所致肠管收缩,减少肠管收缩平均张力、振幅、频率和最大张力(分别与给小半夏汤前和不给小半夏汤相比,P<0.05或P<0.01);小半夏汤能够增大DA致肠管收缩平均张力、振幅和频率(分别与给小半夏汤前和不给小半夏汤相比,P<0.05或P<0.01),但对肠管收缩最小张力无明显影响。 结论: 小半夏汤能够抑制豚鼠离体肠管自主收缩,其作用机制可能是通过阻断M受体、H₁受体、5-HT受体和D₂受体等呕吐相关受体而介导,或直接作用于肠平滑肌而起作用,其止呕机制有待进一步研究。

Effects of XiaoBanXia Decoction on Movement of Isolated Ileum in Guinea Pig

Objective: To observe the effect of Xiaobanxia decoction on the movement of isolated ileum in guinea pig and analyze the mechanism of anti-emetic effect of Xiaobanxia decoction(XBXD). Method: The effects of XBXD(0, 0.1, 0.25, 0.5, 1, 2.5, 5, 10, 20, 40 g·L^-1) on ileum spontaneous contraction tension, amplitude and frequency were investigated in organ bath. Emesis-relating agonists acetylcholine (Ach), histamine (His), 5-hydroxytryptamine (5-HT), dopamine (DA) were used as tool drugs, the inhibitory effect of XBXD (0, 2, 5, 10 g·L^-1) on contraction tension, amplitude and frequency of isolated ileum in guinea pig were observed. Result: XBXD could dose-dependently inhibit ileum spontaneous contractions, reducing the average tension, amplitude, frequency (P<0.05 or P<0.01 vs control). Ach, His and 5-HT could stimulate ileum contraction, enhance the tension, amplitude and frequency. All the three doses (10, 5, 2 g·L^-1) of XBXD could suppress the ileum contraction stimulated by above three tool drugs, significantly reduced the contraction tension, amplitude and frequency (P<0.05 or P<0.01 vs before XBXD or XBXD 0 g·L^-1). DA could inhibit the spontaneous contraction, the middle and small dose (5, 2 g·L^-1) of XBXD could antagonize the inhibitory effect of DA, and enhance the contraction tension, amplitude and frequency (P<0.05 or P<0.01 vs before XBXD or XBXD 0 g·L^-1). Conclusion: XBXD can inhibit spontaneous contractile activity of ileum in guinea pig, the mechanism may be related to blocking M, H₁, 5-HT and D₂receptors or directly suppressing ileum smooth muscle. The mechanism of anti-emetic effect of XBXD needs further study.