中风1号对实验性脑缺血的改善作用

目的: 观察中风1号对小鼠急性脑缺血和大鼠局灶性脑缺血引起的脑梗死的保护作用。 方法: 小鼠按体重均匀分为5组:空白对照组、尼莫地平阳性对照组、中风1号高、中、低剂量组(31.74,15.87,7.94 g·kg^-1),给药8 d后,采用小鼠双侧颈总动脉合并迷走神经结扎法制备小鼠急性脑缺血模型,观察小鼠呼吸维持时间;大鼠按体重均匀分为假手术对照组、模型对照组、尼莫地平组以及中风1号(24.78, 12.39,6.20 g·kg^-1)剂量组,预给药3 d后采用线栓阻断大脑中动脉血供造成大鼠局灶性脑缺血模型(MCAO),观察中风1号对脑缺血大鼠神经功能评分和脑梗死比例的影响。 结果: 中风1号高剂量组能延长急性脑缺血小鼠呼吸维持时间,与模型对照组有显著差异(P<0.05)。与模型对照组比较,中风1号高、中剂量组能不同程度降低局灶性脑缺血大鼠脑梗死率(P<0.001,P<0.01),并能明显改善神经功能损伤症状。 结论: 中风1号对小鼠和大鼠不同脑缺血模型都有很好的作用,能延长小鼠呼吸维持时间,明显缩小大鼠脑缺血梗死率并改善神经损伤症状。

Amelioration Effect of Zhongfeng 1 on Experimental Cerebral Ischemia

Objective: To observe the neuroprotection effect of Zhongfeng 1 on cerebral infarction caused by acute cerebral ischemia in mice and focal cerebral ischemia in rats. Method: Acute cerebral ischemia model in mice was induced by ligaturing the bilateral carotid arteries and vagus nerve, and breathing duration was observed.Cerebral ischemia and infarction in rats were induced by middle cerebral artery occlusion (MCAO). The effects of Zhongfeng 1 were evaluated in the two models. The different doses of Zhongfeng 1 were administrated before modeling (31.74,15.87,7.94 g·kg^-1 and 24.78, 12.39,6.20 g·kg^-1 respectively for mice and rats). In mice, Zhongfeng 1 was given for 8 days before modeling. In rats, Zhongfeng 1 was given for 3 days before modeling. Nimodipine was used as the positive control. Result: Each dose group of Zhongfeng 1 could prolong the breathing duration in mice with acute cerebral ischemia, and the effect was dose-dependent, but without significant difference among different dose groups. Both Zhongfeng 1 high dose group and middle dose group, compared with the model control group, could significantly reduce the proportion of cerebral focal infarction(P<0.001, P<0.01) and significantly improve symptoms of nerve function injury in rats. Conclusion: Zhongfeng 1 has good pharmacological effects on cerebral ischemia in mice and rats. It can prolong the maintenance of mice breathing, significantly reduce infarction ratio of cerebral ischemia, and improve the symptoms of nerve damage.