内标多控法测定枳实、枳壳、青皮和陈皮中黄酮类成分含量

目的:建立内标多控法测定芸香科植物枳实、枳壳、青皮以及陈皮中黄酮类成分的含量。方法:以柚皮苷为内标,建立柚皮苷与橙皮苷、新橙皮苷的相对校正因子,对其进行耐用性考察;采用内标多控法计算芸香科植物中黄酮类成分的含量,并用夹角余弦法对计算值与外标法的实测值进行比较。结果:流速、柱温、色谱柱以及仪器对相对校正因子无显著影响;采用内标多控法计算芸香科植物黄酮类成分的含量与外标法的实测值没有显著差异。结论:所建立的内标多控法可用于枳实、枳壳、青皮以及陈皮中黄酮类成分的定量分析和质量控制。

Determination of Flavone Ingredients in Citrus Aurantium, Bitter Orange Immature, Walnut Peel and Tangerine Peel By Internal Standard Multi-control Method

Objective:To establish the internal standard multi-control method and applied to the determina-tion of flavonoid compounds in rutaceae plant:citrus aurantium,bitter orange Immature,walnut peel and tangerine peel.Method:Naringin as index,establishing the relative correction factor of naringin,hesperidin and neohesperi-din and investigating the durability.Internal standard multi-control method was used to determine the content of fla-vonoid compounds in rutaceae plant,using included angle cosine method to com...