当归对阴虚哮喘小鼠模型的影响

目的:探讨当归对阴虚哮喘BALB/c小鼠的治疗作用。方法:84只BALB/c小鼠随机分为7组,正常组,模型组,地塞米松组(地塞米松,0.5 mg·kg~(-1)),当归高、中、低剂量组(8,4,2 g·kg~(-1))及联合组(当归4 g·kg~(-1)与地塞米松0.5 mg·kg~(-1)联合用药),除正常组外,其余各组通过注射卵清白蛋白(OVA)致敏、雾化吸入OVA激发的方法复制哮喘BALB/c小鼠模型,实验后期ig甲状腺素来复制阴虚哮喘小鼠模型;在当归的干预下观测当归对小鼠体重、肺液清除功能、自主活动、哮喘行为学、肺功能、血清环磷酸腺苷(c AMP)与环磷酸鸟苷(c GMP)及肺脏病理学的影响。结果:与正常组比较,模型组动物肺脏含水量与体重显著下降,小鼠自主活动、进食量与饮水量明显升高,c AMP水平明显升高,c GMP水平明显降低,c AMP/c GMP水平明显升高,小鼠潮气量明显降低,呼吸频率与哮喘行为学综合评分明显升高,肺组织病理学变化较为明显(P0.05,P0.01);与模型组比较,当归高、中、低剂量组及联合组在维持阴虚哮喘模型肺组织含水量与体重的同时可减少小鼠自主活动、进食量与饮水量,降低c AMP水平、提高c GMP含量而缓解c AMP/c GMP失衡状态(P0.05,P0.01),改善阴虚哮喘模型的阴虚症状;当归可增加阴虚哮喘小鼠潮气量,降低呼吸频率与哮喘行为学综合评分,缓解肺组织病理学变化(P0.05,P0.01),改善阴虚哮喘模型的哮喘症状。结论:当归对阴虚哮喘的阴虚证与哮喘有一定的治疗作用。

Effect of Angelicae Sinensis Radix on Asthmatic Mice with Yin Deficiency Syndrome

Objective: To investigate the effects of Angelicae Sinensis Radix (ASR) on asthmatic mice with Yin deficiency syndrome. Method: Totally 84 BALB/c mice were randomly divided into seven groups:normal group, model group, dexamethasone group (dexamethasone, 0.5 mg·kg^-1), ASR high, middle and low-dose groups (ASR, 8, 4, 2 g·kg^-1), and ASR + dexamethasone group (combination group, ASR 4 g·kg^-1 and dexamethasone 0.5 mg·kg^-1). Except for normal group, all of the remaining groups were included into the BALB/c model by injecting ovalbumin (OVA) for sensitization, inhaling OVA for stimulation. In the late stage of the experiment, the asthmatic mouse model with Yin deficiency syndrome was reproduced through gavage with thyroxine; rat body weight, pulmonary fluid clearance, autonomous activity, asthmatic behaviors, respiratory function, cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP), and lung histopathology were observed under the intervention of ASR. Result: Compared with normal group, model group showed significant decreases in water content in lung tissues, body weight, cGMP, tidal volume, and increases in autonomous activity, food and water intake, cAMP, cAMP/cGMP, respiratory frequency and comprehensive asthmatic behavior score, with obvious pathologic changes in lung tissues (P<0.05, P<0.01); compared with normal group, ASR high, middle and low-dose groups can improve Yin deficiency syndrome by maintaining pulmonary fluid clearance and body weight, depressing autonomous activity, food and water intake, cAMP and cAMP/cGMP, and increasing cGMP content, and relieving cAMP/cGMP imbalance status (P<0.05, P<0.01). And it can also alleviate asthma by increasing tidal volume, depressing breathing rate, asthmatic behaviors, bronchial wall epithelial cell exfoliation, and pathologic changes in lung tissues (P<0.05, P<0.01). Conclusion: ASR has a certain effect in treating asthma and asthma with Yin deficiency syndrome.