艾迪注射液抑制肿瘤新生血管形成的实验研究

目的:研究艾迪注射液抑制肿瘤生长的作用机理。方法:采用裸鼠人肺鳞癌移植瘤模型,造模10d后,随机分为3组:对照组每日ip生理盐水0.2mL,共20d。艾迪组按12g·kg^-1每日ip艾迪注射液0.2mL(含生药1.2g·mL^-1),共20d。化疗组按照1mg·kg^-1给小鼠ip0.2mL顺铂(DDP),共7d。给药结束后处死动物,称瘤重;用免疫组化染色检测肿瘤血管密度和VEGF蛋白表达。结果:艾迪注射液和DDP的抑瘤率分别为45.6%和78.3%,艾迪注射液组血管密度降低,肿瘤组织VEGF蛋白表达减少,DDP组血管密度也降低,但肿瘤组织VEGF蛋白表达增加。结论:艾迪注射液可抑制肿瘤组织VEGF蛋白表达,降低肿瘤血管密度。

An Experimental Study of the Anti-angiogenesis Effect of Aidi Injection

Objective:To explore the mechanism of the anti-tumor effect of Aidi Injection.Methods:Human lung squamous cell carcinoma xenografts were transplanted into 30 nude mice.Mice were randomLy divided into 3 groups:The Aidi Injection group received Aidi Injection(12 g·kg^-1),0.2 mL,peritoneal injection) every day for 20 days,the chemotherapy group received DDP(1 mg·kg^-1),0.2 mL peritoneal injection) every day for 7 days,and the control group received normal sodium(0.2 mL peritoneal injection) every day for 20 days.They were sacrificed afterwards and their tumors were processed for histological examination.The weights of the tumors were measured,the vessel density of the tumor and the expression of VEGF in tumors were detected using immunohistochemical method.Results:The cancer inhibition rates of Aidi Injection and DDP are 45.6% and 78.3% accordingly.In the Aidi Injection group,the vessel density of the tumor and the expression of VEGF in tumors decreased.In the DDP group,the vessel density of the tumor decreased,while the expression of VEGF in tumors increased.Conclusion: Aidi Injection can inhibit the expression of VEGF in tumor and decrease the vessel density of the tumor.