GC-MS考察羌活、独活及其药对中挥发油 透皮成分及透皮效果

目的:分别对羌活、独活及其药对中挥发油的透皮成分进行分析,同时比较其透皮效果.方法:采用GC-MS鉴定挥发油体外透皮供给液与接收液中的化学成分,根据面积归一化法计算各成分相对含量.通过数学推导将相对含量转化为透过率,从而反映各成分透皮效果.结果:透皮后鉴定出羌活39个成分、独活29个成分、药对36个成分,分别占挥发油总量的93.22％,93.74％,92.75％.1-松油醇(30.96％),4-松油醇(28.22％),冰片(7.10％)等是羌活挥发油的主要透皮成分；邻-乙酰基-对-甲基苯酚(55.70％),反-氧化芳樟醇(6.79％),1,7,7-三甲基二环2.2.1]5-己烯-2-酮(4.78％)等是独活挥发油的主要透皮成分；1-松油醇(28 86％),4-松油醇(22.41％),邻-乙酰基-对-甲基苯酚(12.15％),冰片(5.62％)等是药对挥发油的主要透皮成分.结论:3种挥发油中各成分透过率可定性地对其透皮效果进行排序.其中羌活挥发油中1-松油醇的透过率最高,独活挥发油中1,7,7-三甲基二环2.2.1]5-己烯-2-酮的透过率最高；药对挥发油中1-松油醇的透过率最高.

Investigation of Transdermal Effect and Transdermal Composition of Volatile Oil from Notopterygium incisum, Angelica pubescens and Pair of Them by GC-MS

Objective: To analyze transdermal effect and transdermal composition of volatile oil from Notopterygium incisum,Angelica pubescens and pair of them by GC-MS. Method: Chemical constituents of volatile oil in in vitro transdermal donor cell and receiving cell were identified by GC-MS,The relative contents in percentage were calculated with area normalization method.According to mathematical derivation,relative contents was changed into translation rate,which reflected transdermal effect of every components. Result: After percutaneous absorption, thirty-nine components were identified in N. incisum (93.22%),twenty-nine in A. pubescens (93.74%) and thirty-six in drug pair of them (92.75%).Main components of percutaneous absorption in N. incisum were 1-terpineol (30.96%),4-terpineol (28.22%),borneol(7.10%),etc;Main components of percutaneous absorption in A. pubescens were o-acetyl-p-methylbenzyl alcohol (55.70%), trans-oxy-linalool (6.79%),1,7,7-trimethyl-bicyclic 5-hexene-2-one(4.78%),etc;Main components of percutaneous absorption in drug pair of them were 1-terpineol (28.86%), 4-terpineol (22.41%), o-acetyl-p-methylbenzyl alcohol (12.15%), borneol (5.62%),etc. Conclusion: Transmission rate of every components in three kinds of volatile oil could be sorted qualitatively of transdermal effect.Transmission rate of 1-terpineol was the most in volatile oil of N. incisum. 1,7,7-trimethyl-bicyclic 5-hexene-2-one had the most transmission rate in volatile oil of A. pubescens. 1-terpineol had the most transmission ratio in volatile oil of drug pair of them.