无梗五加果实中齐墩果酸苷的分离与鉴定

目的:研究无梗五加Acanthopanax sessiliflorus( Ruqr.et Maxim) Seem.果实的化学成分.方法:采用溶剂提取法、溶剂萃取法、硅胶柱色谱法、凝胶柱色谱法、重结晶法对无梗五加果实中的化合物进行分离纯化；根据光谱数据和理化性质确定化合物结构.结果:分离并鉴定了6个化合物,分别为齐墩果酸-3-O-β-D-葡萄醛酸甲酯苷(1),齐墩果酸-3 -O-α-L-阿拉伯糖苷(2),齐墩果酸-3-O-β-D-葡萄醛酸正丁酯苷(3),齐墩果酸-3-O-β-D-葡萄糖醛酸苷(4),齐墩果酸(5),3-0-[(α-L-arabinopyranosyl) -( 1→2)] -[ -β-D-glucuronopyranosyl-6-O-methyl ester] -olean-12-ene-28-olic acid(6).结论:化合物2,3为首次从无梗五加果实中分离得到.

Isolation and Identification Glycosides of Oleanolic Acid from Fruits of Acanthopanax sessiliflorus

Objective:To study the chemical constituents of the fruits of Acanthopanax sessiliflorus.Method:The methods of solvent extraction,silica gel column chromatography,Sephadex LH-20 column chromatography and recrystallization were used for the isolation;spectroscopy was used for the structure identification.Result:Six compounds were isolated and identified as oleanolic acid 3-O-6′-methyl-β-D-glucuronopyranoside(1),3-O-α-L-arabinopyranosyl-oleanolic acid(2),oleanolic acid 3-O-6′-buthyl-β-D-glucurono-pyranoside(3),momordin lb(4),oleanolic acid(5),3-O-[(α-L-arabinopyranosyl)-(1→2)]-[-D-glucuronopyranosyl-6-O-methyl ester]-olean-12-ene-28-olic acid(6).Conclusion:Compounds 2,3 were first isolated from the fruits of A.sessiliflorus.