硫磺熏蒸山药对大鼠肝组织抗氧化能力及ATP酶活性的影响

目的:观察硫磺熏蒸山药对大鼠肝组织内抗氧化能力,Ca2+Mg2+-ATPase,Na+K+-ATPase活性的变化。方法:将实验动物随机分为4组,其中3组分别给予未熏蒸山药、市售品山药、硫磺熏蒸山药水提浓缩液(5 g.mL-1),掺入基础饲料,50g.kg-1 BW;正常对照组给予基础饲料,测定14周后大鼠肝组织中MDA含量、总超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力,Ca2+Mg2+-ATPase,Na+K+-ATPase活性;同时观察肝组织结构的病理变化。结果:经口给予受试物14周,两性动物未薰山药组、市售山药组肝组织中GSH-PX活力与对照组比较均显著增加,均有显著性差异(P0.05或P0.01);市售山药组雌性大鼠肝组织内Na+K+-ATPase活性比对照组高,有显著性差异(P0.05)。结论:长期服用经大量硫磺薰制后的山药可能影响肝组织的氧化应激系统和Na+K+-ATPase过程。

Effect of Sulfur Fumigated Dioscorea opposita on Oxidative Stress and ATP Enzyme in Liver of Rats

Objective: To investigate the influence of Sulfur fumigated Dioscorea opposita on antioxidation,Ca²⁺Mg²⁺-ATPase,Na⁺K⁺-ATPase in liver of rats. Method: Wistar rats were divided randomLy into 4 groups, one control and three experimental groups administrated diets mixed with concentrated extractions from unfumigated, commercial and sulfur fumigated D. opposita respectively. The activities of Ca²⁺Mg²⁺-ATPase and Na⁺K⁺-ATPase and the level of MDA and activities of SOD and GSH-Px 14 weeks after treatment were measured and observe liver framework of organization change. Result: Results showed that activities of GSH-Px 14 weeks after treatment of unfumigated, commercial D. opposita were significantly increased compared with control group; commercial D. opposita enhanced the activities of Na⁺K⁺-ATPase. Conclusion: The long-term use of D. opposita has the possibility to cause lipid peroxidation and change the activities of Na⁺K⁺-ATPase in liver.