黄芪甲苷配伍姜黄素对人卵巢癌HO-8910原位移植瘤转移的抑瘤作用

目的:研究黄芪甲苷配伍姜黄素对人卵巢癌HO-8910原位移植瘤出现转移的抑瘤作用,探讨两者配伍对抗肿瘤是否有协同增效作用。方法:选取已建立荧光蛋白转染的HO-8910卵巢癌动物模型,设G1模型组,G2顺铂组,G3黄芪甲苷组,G4姜黄素组,G5黄芪甲苷+姜黄素配伍组,每组8只。称瘤重并计算抑瘤率;免疫组化法检测肿瘤组织基质金属蛋白酶2(matrix metallopeptidase 2,MMP2),B细胞淋巴瘤/白血病-2(apoptosis regulator,Bcl-2)的蛋白表达;实时荧光定量PCR(Realtime PCR)法检测MMP2,Bcl-2及miR21,miR15a,miR200a基因表达。结果:荷瘤鼠经治疗后,与模型组比较,瘤重均有所减小,配伍组瘤重明显低于其他组(P0.05)。免疫组化结果显示,与模型组比较,顺铂组、单体组MMP2,Bcl-2蛋白表达均降低,配伍组明显降低(P0.05);Real-time PCR结果显示,与模型组比较,中药组MMP2,Bcl-2,miR21基因表达均降低,配伍组明显下调(P0.05),miR15a,miR200a基因表达均增强,配伍组明显上调(P0.05)。结论:黄芪甲苷配伍姜黄素对人卵巢癌HO-8910原位移植瘤出现转移后有抑瘤作用,其作用机制可能与抑制MMP2,Bcl-2,miR21表达,上调miR15a,miR200a表达有关,黄芪甲苷配伍姜黄素确有协同增效作用。

Anti-tumor Effect of Astragaloside Combined with Curcumin on Orthotopic Transplantation Tumor Tissue of HO-8910 Ovarian Carcinoma Metastasis

Objective: To study the anti-tumor effect of astragaloside combined with curcumin on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis and discuss whether the combination of astragalosideand curcumin has the synergies effect against tumor. Method: HO-8910 ovarian carcinoma animal models withfluorescent protein transfectionwere selected,including G1 model group, G2 Cisplatin group, G3 Astragaloside group, G4 curcumin group, G5 Astragaloside+curcumin group, n=8 in each group. The tumor was weighted and the inhibitory rate was calculated. The protein expression levelsof matrix metallopeptidase 2 (MMP2) and apoptosis regulator (Bcl-2) were detected by immunohistochemical method, and thegene expression levels of MMP2, Bcl-2, miR21, miR15a and miR200a were detected by Real-time PCR. Result: Tumor weights of the tumor-bearing nude mice after treatment were lower than those in model group; the tumor weight in Astragaloside+curcumin group was significantly lower than that in other groups(P<0.05).Immunohistochemical results showed that the levels of the protein expressions of MMP2,Bcl-2 in G2, G3 and G4 groups were lower than those in model group, and the levels in G5 group were significantly reduced (P<0.05). Real-time PCR results showed that the gene expression levels of MMP2, Bcl-2 and miR21 in Chinese medicine groups were lower than those in model group, and the levels in G5 groupweresignificantly reduced (P<0.05); the gene expression levels of miR15a, miR200a were higher than those in model group, and the levels in G5 group were significantly increased (P<0.05). Conclusion: The combination of astragaloside and curcumin has the suppression effect on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis, which may be associated with the down-regulation of MMP2, Bcl-2 and miR21 expressions and the up-regulation of miR15a and miR200a expressions. Therefore, the combination of astragaloside and curcumin has the synergies effect against tumor.