牛黄上清微丸提取工艺研究

目的:优选牛黄上清微丸提取方法。方法:采用L9(34)正交实验,以大黄素、大黄酚及黄芩苷为指标评价大黄和黄芩的醇提取工艺;采用L9(34)正交实验,以栀子苷为指标评价栀子等其余药物水提取工艺。结果:大黄、黄芩的醇提取部分最佳工艺为加5倍量70%乙醇回流提取3次,每次1.5 h;栀子等其余药物水提取最佳工艺为加10倍量水,提取2次,每次1 h。结论:提取工艺设计合理,方法可行,对大生产有指导意义。

Extraction Method of Niuhuang Shangqing Pellets

Objective: To establish the best extraction method of Niuhuang Shangqing pellets. Method: L₉ (3⁴) orthogonal experiment was adopted to optimize the alcohol extraction process of rhubarb and baicalin with the yeild of emodin, chrysophanol and baicalin as evaluation target; L₉ (3⁴) orthogonal experiment was adopted to optimize the water extraction process of the rest herbs with the yeild of geniposide as evaluation target. Result :Optimum alcohol-extracting factors were 5 times of 70% alcohol, circum-fluence extracting for 1.5 h, for 3 times; Optimum water-extracting factors were 10 times of water, refluxing and extracting for 1h, for 2 times. Conclusion : The extraction method was designed reasonable and it was instructive to industrial production.