高效液相色谱法测定昆仑雪菊中绿原酸和黄芩苷的含量

目的:建立新疆昆仑雪菊中绿原酸和黄芩苷的HPLC含量测定方法。方法:采用Inertsil ODS-SP色谱柱(4.6 mm×150 mm,5μm);流动相甲醇-0.05%磷酸水溶液(三乙胺调pH为3.0)梯度洗脱;流速0.6 mL.min-1,检测波长330nm,柱温25℃。结果:绿原酸线性回归方程为A=55 102C-24 808,r=0.999 8(n=6),线性范围为0.45～15.00 mg.L-1,平均回收率为102.58%.(RSD 0.93%,n=9);黄芩苷回归方程为A=25 599C-16 099,r=0.999 7(n=6),线性范围为0.90～30.00 mg.L-1,平均回收率为104.90%(RSD 1.12%,n=9)。结论:本方法稳定,重复性好,操作简单,是检测新疆昆仑雪菊中绿原酸与黄芩苷的较理想方法。

Determination of Chlorogenic Acid and Baicalin in Kunlun Chrysanthemum by HPLC

Objective:To establish a HPLC method for the determination chlorogenic acid and baicalin in Kunlun Chrysanthemum.Method:The HPLC method was used.The sample was analyzed on an Inertsil ODS-SP(4.6 mm × 150 mm,5 μm) column at 25 temperature,with mobile phase consisting of methanol and 0.05% phosphoric acid(pH 3.0) by gradient eluteion at the flow rate of 0.6 mL.min-1,The wavelength was at 330 nm.Result:The regression equation of chlorogenic acid was linear in the range of 0.45-15.00 mg.L-1,A =55 102C-24 808,r = 0.999 8(n = 6).The average recovery was 102.58%(RSD 0.93%).The regression equation of baicalin was linear in the range of 0.90-30.00 mg.L-1,A = 25 599C-16 099,r = 0.999 7(n = 6).The average recovery was 104.90%,(RSD 1.12%).Conclusion:The method was stable and accurate,it is suitable to determine chlorogenic acid and baicalin in Kunlun Chrysanthemum