银杏叶内酯N对实验性大鼠脑缺血再灌注损伤的保护作用

目的:探讨银杏叶内酯N( ginkgolide N,GN)对实验性大鼠大脑局灶性脑缺血再灌注损伤的保护作用.方法:采用栓线法阻断大鼠大脑中动脉缺血2h后再灌注22 h模型,探讨银杏内酯N对模型大鼠神经缺损症状、脑梗死百分比、脑组织含水率、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及大脑皮层神经元细胞内Ca2+];水平影响.结果:与假手术组相比,缺血再灌注组神经缺损评分、含水率、梗死率、脑组织MDA含量增加、SOD活性降低及Ca2+]；显著升高(P＜0.01)；与脑缺血再灌注组相比,GN高、中、低剂量组,阳性对照组脑组织MDA含量减少、SOD活性升高、Ca2+]；显著下降(P＜0.01或P ＜0.05),GN高、中剂量组,阳性对照组大鼠神经缺损评分、脑含水率、脑梗死率显著降低(P＜0.01)；GN各剂量组间结果亦有显著差异(P＜0.01或P＜0.05).结论:GN对局灶性脑缺血再灌注损伤大鼠具有保护作用,可能与其减少大鼠脑缺血再灌注后脑组织Ca2+]；浓度、抗自由基损伤有关.

Protective Effects of Ginkgolide N on Focal Cerebral Ischemia Reperfusion Injury in Rats

Objective: To investigate the protective effect and the mechanism of ginkgolide N (GN) model of focal cerebral ischemia reperfusion injury in rat. Method: The model of ischemia (2 hours) and reperfusion (22 hours) induced by thread approach was reproduced for evaluating the effect of GN on the neurological symptoms, infarction area percentage, rate of brain water, content of malondialdehyde (MDA), activitiy of SOD in the brain tissues and the intracellular _i in the cerebral cortex. Result: Compared with I/R group, GN high dosage group, middle dosage group and nimodipine group relieved signfecantly the neurological symptoms and reduced the cerebral infarction area percentage and content of brain water in rats, meanwhile all GN and nimodiping groups ameliorated the activity of SOD and decreased MDA content andCa²⁺]_i level after ischemia and reperfusion injury. Conclusion: GN has the protective effect on rats with cerebral focal ischemia and reperfusion injury, which may be related to that GN can reduce the content of brain water, infarction area percentage, content of MDA andCa²⁺]_i level and increase the activity of SOD.