博落回提取物对实验性肝纤维化的防治作用

目的:观察博落回提取物(Macleaya cordata extract,MCE)对实验性肝纤维化的防治作用.方法:采用CCl4复合因素诱导的大鼠肝纤维化和小鼠血吸虫肝纤维化2种模型,观测MCE干预后肝指数、肝功能生化指标丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)]、血清纤维化指标Ⅲ型前胶原( PCⅢ)、层黏连蛋白(LN)、透明质酸(HA))、脂质过氧化指标谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)),肝组织Ⅰ型胶原(CoⅠ)、Ⅲ型胶原(CoⅢ)及羟脯氨酸(Hyp)表达的变化,并做肝脏病理学检查.结果:在CCl4肝纤维化大鼠模型中,MCE各剂量预防组肝指数,AST含量及CoⅠ表达明显降低,高剂量预防组LN及CoⅢ表达明显降低；中剂量预防组MDA含量及CoⅢ表达明显降低；MCE治疗组肝指数、HA,MDA含量及CoⅢ表达明显降低；经MCE防治后大鼠肝纤维化程度有不同程度减轻.在小鼠血吸虫肝纤维化模型中,MCE预防组小鼠Hyp及Co Ⅰ表达明显降低；高剂量治疗组小鼠肝指数、PCⅢ,LN,HA,AST,ALT含量及Co Ⅰ表达显著降低,中剂量治疗组PCⅢ,HA,AST,ALT含量明显降低；MCE治疗给药能明显改善模型小鼠肝脏病变.结论:MCE对实验性肝纤维化有一定的防治作用,其机制可能与其保护肝细胞膜、减轻肝脏炎症及抗脂质过氧化作用有关.

Experimental Study on Antagonizing Liver Fibrosis of Macleaya cordata Extract

Objective:To investigate the preventive and therapeutic effects of Macleaya cordata extract(MCE) on experimental liver fibrosis.Method:Experimental liver fibrosis models were induced by injection of carbon tetrachloride in combination with the drinking of 5% alcohol on rats and Schistosoma japomicum infection in mice.The effect of MCE on anti-hepatic fibrosis was evaluated by comparing the liver index,tissue biochemical indices(ALT,AST),lipid peroxidation indices(GSH-Px,MDA,SOD),serum fibrosis indices(HA,PCⅢ,LN),the expression levels of Hyp and collagen type Ⅰ,Ⅲ(CoⅠ,CoⅢ),the liver pathology before and after MCE intervention.Result:In CCl4-induced liver fibrosis model in rats,Compared with model group,the liver index,the level of AST,the expression of CoⅠwere obviously decreased in MCE preventive groups,and high-dose preventive MCE could significantly reduce the content of LN and the expressionof CoⅢ,middle-dose preventive MCE could significantly reduce the content of MDA and the expression of CoⅢ.The liver index,the contents of HA,MDA,the expression of CoⅢ were significantly decreased in MCE therapeutic group;in S.japomicum-induced liver fibrosis model in mice,preventive MCE could significantly reduce the content of ALT,the expression of Hyp and CoⅠ;the liver index,the contents of PCⅢ,LN,HA,AST,ALT in serum and the expression of CoⅠwere significantly decreased in high-dose MCE therapeutic group,and middle-dose therapeutic MCE could significantly reduce the levels of PCⅢ,HA,AST,ALT.In addition,the hepatic histopathology were also improved in varying degrees after MCE intervention.Conclusion:MCE has preventive and therapeutic effects on experimental liver fibrosis by inhibiting hepatic lipid peroxidation and alleviating the inflammation.