附子理中汤对脾阳虚大鼠AQP4的影响及其止泻机制

目的:观察附子理中汤对脾阳虚大鼠水通道蛋白4(AQP4)的影响,探讨附子理中汤止泻作用的机制.方法:Wistar大鼠100只随机分为对照组、模型组、附子理中汤低、中、高剂量组5组,每组20只.在模型组基础上,低剂量组按10g·kg-1ig,中剂量组按20 g·kg-1 ig,高剂量组按40 g·kg-1 ig,每天1次,连续4周.取结肠标本,酶联免疫法(ELISA)法检测AQP4含量,RT-PCR法检测AQP4 mRNA表达.结果:和对照组比较,模型组的AQP4含量(13.25 ±4.22) ng·L-1.AQP4mRNA相对表达量(0.38±0.11)均降低(P＜0.01或P＜0.05).和模型组比较,附子理中汤中剂量组的AQP4含量(23.84±5.68) ng·L-1,AQP4 mRNA相对表达量(0.54±0.26)均升高(P＜0.05).和模型组比较,高剂量组的AQP4含量(28.98±6.12) ng·L-1,AQP4 mRNA相对表达量(0.62±0.32)均显著升高(P＜0.01).结论:附子理中汤能恢复脾阳虚大鼠AQP4含量和AQP4 mRNA正常表达,以高剂量的恢复作用较明显,附子理中汤可能通过上调AQP4表达,促进胃肠道黏膜对水液的重吸收而起到止泻的作用.

Effect of Fuzi Lizhong Decoction on AQP4 in Rats with Spleen Yang Deficiency Syndrome and its Antidiarrheal Mechanism

Objective: To observe effect of Fuzi Lizhong decoction on aquaporin 4(AQP4) in rats with spleen Yang deficiency syndrome and discuss its antidiarrheal mechanism. Method: One hundred Wistar rats were randomly divided into control group,model group, low-dose group, middle-dose group and high-dose group,20 rats each group. low-dose group, middle-dose group and high-dose group were ig given with Fuzi Lizhong decoction according to 10,20,40 g·kg^-1 based on model group, once a day for four weeks.colon was collected as sample to examine content of AQP4 by ELISA method and expression of AQP4 mRNA by RT-PCR method. Result: Compared with control group, in model group both content of AQP4(13.25±4.22) ng·L^-1 and expression of AQP4 mRNA (0.38±0.11)reduced (P<0.01 or P<0.05). Compared with model group, in middle-dose group both content of AQP4 (23.84±5.68)ng·L^-1and expression of AQP4 mRNA (0.54±0.26)increased (P<0.05). Compared with model group, in high-dose group both content of AQP4 (28.98±6.12)ng·L^-1and expression of AQP4 mRNA (0.62±0.32)increased significantly (P<0.01). Conclusion: Fuzi Lizhong decoction could recover content of AQP4 and expression of AQP4 mRNA, and high dose had a better effect. Fuzi Lizhong decoction could up-regulate expression of AQP4 mRNA and promote gastrointestinal reabsorption of water,which perhaps are one of its antidiarrheal mechanisms.