益肾泻浊化瘀方治疗大鼠慢性肾功能衰竭的实验研究

目的 :观察益肾泻浊化瘀方对慢性肾功能衰竭(CRF)大鼠肾脏的保护作用,并探讨其作用机理。 方法 :将大鼠随机分为中药益肾泻浊化瘀方组、西药依那普利组、模型组及假手术组,以5/6肾大部切除术建立慢性肾衰大鼠模型,检测各组治疗前后血尿素氮(BUN)、血肌酐(Cre);血一氧化氮(NO)、内皮素(ET)、降钙素基因相关肽(CGRP)水平及肾组织病理改变。 结果 :治疗后中药组和西药组BUN、Cre均较模型组明显下降(P<0.01),且中药组Cre下降幅度较西药组大(P<0.05)。模型组大鼠血ET、CGRP含量较假手术组显著升高(P<0.01),中药、西药均能有效降低ET水平,与模型组比较分别为P<0.01和P<0.05,中药组血CGRP含量下降(P<0.05)。各组血NO水平无显著差异。模型组肾组织病理改变最严重,中药组较西药组病变程度轻。 结论 :益肾泻浊化瘀方能有效保护CRF大鼠肾功能,且疗效优于西药依那普利,可能与其降低CRF大鼠血ET水平,减轻受损肾脏的病理改变等有关。

The Experimental Study of Yishen Xiezhuo Huayu Decoction on Chronic Renal Failure in Model Rats

Objective: To investigate the effects of Yishen Xiezhuo Huayu Decoction on chronic renal failure (CRF) in model rats and explore its action mechanism. Methods: Rats were randomly divided into the traditional Chinese medicine(TCM) Yishen Xiezhuo Huayu Decoction group, the Western medicine enalapril maleate group, the model group and the sham group. The CRF model was induced by 5/6 subtotal nephrectomy in rats. The blood levels of urea nitrogen(BUN), creatinine(Cre), nitric oxide (NO), endothelin (ET),clacitonin gene-related peptide (CGRP) and pathological changes of residual kidney were detected. Results: Following treatment, the blood levels of BUN and Ser of both the TCM group and the Western medicine group significantly declined compared with those of the model group (P<0.01); moreover, the decline in the TCM group was more remarkable than in the western medicine group (P<0.05). Compared with the sham group, the blood levels of ET and CGRP of the model group significantly rose (P<0.01). Both the drugs studied could effectively lower the blood level of ET and the decreasing extents were significant in both the TCM group (P<0.01) and the Western medicine group (P<0.05) when compared with the model group. The blood level of CGRP of the TCM group also declined (P<0.05). There were no significant differences in the blood level of NO of each group. The most severe pathologic changes in renal tissue were observed in the model group and Yishen Xiezhuo Huayu Decoction could alleviate the changes more effectively than enalapril maleate. Conclusion: Yishen Xiezhuo Huayu Decoction can protect renal functions of CRF rats more effectively than enalapril maleate. The action mechanism of the TCM might include the down-regulation of the blood ET level, and the alleviation of pathologic changes of renal tissues.