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火绒草中总黄酮的纯化工艺优选
引用本文:曹跃,王丽,周翎,谢雪,许枬.火绒草中总黄酮的纯化工艺优选[J].中国实验方剂学杂志,2012,18(10):38-40.
作者姓名:曹跃  王丽  周翎  谢雪  许枬
作者单位:1. 辽宁中医药大学药学院,大连,116600
2. 大连海港医院,大连,116101
基金项目:十一五"重大新药创制"科技重大专项项目(2009ZX09103-394)
摘    要:目的:优选火绒草中总黄酮的大孔吸附树脂富集纯化工艺.方法:采用静态吸附/解吸与动态吸附/解吸相结合的方法,用紫外-可见分光光度法测定火绒草中总黄酮含量,筛选最佳大孔吸附树脂,并对其纯化工艺进行优选.结果:确定采用HPD100型大孔吸附树脂对火绒草中总黄酮进行富集纯化,其最佳工艺条件为以2 BV火绒草供试品溶液(生药质量浓度0.1 g·mL-1)为最大上样量,吸附速率为1 BV·h-1,吸附2h,洗脱流速4 BV·h-1,5 BV水洗脱,4 BV 30%乙醇洗脱,3 BV 50%乙醇洗脱,收集洗脱液,减压浓缩,即得.按上述最佳纯化工艺总黄酮洗脱率>90%.结论:该优选工艺稳定可行,适合工业化生产.

关 键 词:大孔吸附树脂  火绒草  水溶性黄酮  纯化工艺
收稿时间:2012/1/12 0:00:00

Optimization of Purification Technology for Total Flavonoids from Leontopodium leontopodioid
CAO Yue,WANG Li,ZHOU Ling,XIE Xue and XU Nan.Optimization of Purification Technology for Total Flavonoids from Leontopodium leontopodioid[J].China Journal of Experimental Traditional Medical Formulae,2012,18(10):38-40.
Authors:CAO Yue  WANG Li  ZHOU Ling  XIE Xue and XU Nan
Institution:College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;Dalian Harbour Hospital, Dalian 116101, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
Abstract:Objective: To optimize enrichment and purification technology of total flavonoids from Leontopodium leontopodioid by macroporous resin. Method: Optimum macroporous resin and its purification technology was optimized by static adsorption/desorption and dynamic adsorption/desorption.The content of total flavonoids was determined by ultraviolet and visible spectrophotometry method. Result: HPD100 macroporous resin showed good concentration and purification capabilities to total flavonoids from L. leontopodioid. Optimum technology conditions were as follows: 2 BV sample liquid (the concentration of raw drug was 0.1 g·mL-1) of L. leontopodioid was passed through HPD-100 resin in 2 h with adsorption rate of 1 BV·h-1,eluted by 5 BV water, 4 BV 30% ethanol, 3 BV 50% ethanol,respectively, elution flow rate was 4 BV·h-1.Eluate was collected to concentrating under vacuum.Under this optimized technology,the elution rate of total flavonoids was more than 90%. Conclusion: This optimized technology was stable,feasible,it was suitable for industrial production.
Keywords:macroporous resin  Leontopodium leontopodioid  water-soluble flavonoids  purification technology
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