首页 | 本学科首页   官方微博 | 高级检索  
检索        

淫羊霍苷对HCY诱导的血管内皮细胞内质网应激的影响
引用本文:魏晏,沈晓君.淫羊霍苷对HCY诱导的血管内皮细胞内质网应激的影响[J].中国实验方剂学杂志,2010,16(7):147-150.
作者姓名:魏晏  沈晓君
作者单位:1. 河南中医学院第三附属医院,郑州,450008
2. 河南中医学院,郑州,450008
基金项目:河南省科技攻关计划项目 
摘    要:目的:研究淫羊藿苷(icariin,ICA)对同型半胱氨酸(homocysteinemia,HCY)诱导的血管内皮细胞内质网应激的影响,探讨ICA抗动脉粥样硬化的机制。方法:建立HCY诱导的兔血管内皮细胞损伤模型,与不同浓度的ICA共同培养48h后,TUNEL法检测细胞凋亡率;RT-PCR检测葡萄糖调节蛋白78(glucose regulated protein78,GRP78)基因表达;目的基因克隆、鉴定并测序。结果:荧光显微镜观察显示HCY模型组可见多数凋亡细胞,ICA组细胞未见明显凋亡形态学改变;ICA处理组细胞GRP78mRNA表达明显减少,与HCY组比较差异显著(P0.01);测序结果表明,来源于血管内皮细胞的全长648bp的基因片断与兔GRP78基因高度同源。结论:ICA拮抗HCY诱导的血管内皮细胞凋亡,减缓血管内皮细胞内质网应激可能是其作用机制之一。

关 键 词:淫羊霍苷  同型半胱氨酸  血管内皮细胞  内质网应激
收稿时间:2010/1/27 0:00:00

Effects ofICA on HCY-induced Vascular Endothelial Cells Endoplasmic Reticulum Stress
WEI Yan and SHEN Xiao-jun.Effects ofICA on HCY-induced Vascular Endothelial Cells Endoplasmic Reticulum Stress[J].China Journal of Experimental Traditional Medical Formulae,2010,16(7):147-150.
Authors:WEI Yan and SHEN Xiao-jun
Institution:No.3 Affiliated Hospital of Henan College of Traditional Chinese Medicine ,Zhengzhou 450008,China;Henan College of Traditional Chinese Medicine ,Zhengzhou 450008,China
Abstract:Objective: To study the effects of ICA on HCY-induced vascular endothelial cells endoplasmic reticulum stress, and to explore ICA anti-atherosclerotic mechanisms. Method: HCY-induced rabbit endothelial cell injury models were established, and co-cultured with different concentrations of ICA for 48 hours, then apoptosis rate was assayed by TUNEL ; Glucose-regulated protein 78 (GRP78) gene expression was done by RT-PCR detection; The wanted gene was cloned, identified and sequenced. Result: The fluorescence microscope observation showed that there were majority of apoptotic cells in HCY model group, and in ICA group there was no significant morphological changes of apoptosis. Compared with HCY group, cell GRP78 mRNA expression in ICA treatment group significantly reduced (P<0.01). Sequencing results showed that full-length 648bp gene fragment derived from vascular endothelial cells was highly homologous with rabbit GRP78 gene. Conclusion: ICA can resist HCY-induced endothelial cell apoptosis and slow down vascular endothelial cells endoplasmic reticulum stress, which may be one of the mechanisms of its role.
Keywords:icarinn  homocysteine  vascular endothelial cells  endoplasmic reticulum stress
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国实验方剂学杂志》浏览原始摘要信息
点击此处可从《中国实验方剂学杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号