葛根芩连汤对动脉粥样硬化伴随牙周炎大鼠的影响

目的:讨论葛根芩连汤对动脉粥样硬化伴随牙周炎大鼠模型的治疗效果。方法:将40只健康雄性Wistar大鼠,分为健康正常组和模型组,正常组10只,模型组30只,正畸结扎丝结扎于左上颌第二恒磨牙牙颈部龈沟内,高脂饮食辅助药物诱导造模,造模成功后分为模型组、甲硝唑治疗组(0.05g·kg-1·d-1)、阿伐他汀组(0.08g·kg-1·d-1)、甲硝唑联合阿伐他汀组、葛根芩连汤组(0.42g·kg-1·d-1),每组6只,ig给予相应药物,4周后,HE染色光镜下观察治疗前后牙周组织和腹主动脉病理变化,检测治疗前后牙周袋深度及附着丧失检测和血脂总胆固醇(TC),甘油三脂(TG),低密度脂蛋白(LDL),高密度脂蛋白(HDL)水平的改变。结果:与正常组比较,模型组牙周袋深度及附着丧失和血脂TC,TG,LDL水平明显升高(P<0.05),HDL明显降低(P<0.05);与模型组比较,甲硝唑组、阿伐他汀组、甲硝唑联合阿伐他汀组、葛根芩连汤组牙周袋深度及附着丧失和血脂TC,TG,LDL水平均较模型组显著降低,差异有统计学意义(P<0.05),HDL明显升高(P<0.05);甲硝唑联合阿伐他汀组与葛根芩连汤组比较牙周袋深度及附着丧失和血脂TC,TG,LDL,HDL水平无差异。HE染色观察发现牙周炎症破坏明显,结合上皮与牙面分离,上皮向根方增殖,组织深部可见大量炎症细胞浸润,骨小梁稀疏,牙槽骨表面可见骨吸收,牙槽嵴顶高度降低,呈垂直吸收,牙周膜纤维粗细不均或断裂,排列紊乱,各给药组均有不同程度的改善,尤其是甲硝唑联合阿伐他汀组与葛根芩连汤组更为明显。结论:葛根芩连汤能够改善模型大鼠牙周炎症水平并且能够降低大鼠血脂水平,提示该药对牙周炎伴动脉粥样硬化疾病有良好的治疗效果。

Effect of Gegen Qinlian Tang in Periodontitis Combined Atherosclerosis Rat Model

Objective: To discuss the effect of Gegen Qinlian Tang on periodontitis and atherosclerosis rats. Method: The rat model was induced by ligating the second molars of upper left jaw, feeding high fat diet and medications treatment as a complementary method. Thirty successful model rats were divided into the model group, the metronidazole treatment group (0.05 g · kg^-1 · d^-1), the atorvastatin group (0.08 g · kg^-1 · d^-1), the metronidazole combined with atorvastatin group, and the Gegen Qinlian Tang group (0.42 g · kg^-1 · d^-1) of 6 rats in each group. Another 10 rats were assigned to the normal group and feeding with the common forage. After 4-week treatment of the corresponding drug, the changes of periodontal tissue and abdominal aortic pathology were observed under light microscope, HE staining, respectively. The periodontal pocket depth and attachment loss detection, changes of blood lipid total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) were detected. Result: Compared with the normal group, periodontal pocket depth and attachment loss, levels of lipid TC, TG, LDL increased significantly (P<0.05), HDL level decreased significantly (P<0.05) in the model group. Compared with the model group, periodontal pocket depth and attachment loss, and lipid TC, TG, LDL levels decreased significantly (P<0.05), HDL increased significantly (P<0.05) in all treatment groups. No significant difference was found between metronidazole combined with atorvastatin group and Gegen Qinlian Tang group. HE staining showed the destroyed periodontal inflammation, the separation of junctional epithelium and tooth surface, and the proliferation from epithelial to root. The deep tissue showed a large amount of inflammatory cell infiltration and bone trabecular sparse, alveolar bone surface visible bone resorption, alveolar ridge crest height reduced vertical absorption, periodontal ligament fibroblasts dimensional uneven thickness or fractured, arrangement disorder in the model group. These results had good improvement after treatment, and the results were better in the metronidazole combined with atorvastatin group and Gegen Qinlian Tang group. Conclusion: Gegen Qinlian Tang could improve the periodontal inflammation levels and reduce the blood lipid level of model rats. The results suggest that Gegen Qinlian Tang has a good therapeutic effect on periodontitis associated with atherosclerosis disease.