藁本内酯的镇痛抗炎作用

目的:研究藁本内酯的镇痛、抗炎作用。方法:以昆明种小鼠(18～22 g)和SD大鼠(180～220 g)为试验动物,研究100,60,20 mg·kg-1藁本内酯ig给药对受试动物的镇痛和抗炎作用。采用热板法和醋酸致小鼠扭体反应,进行镇痛实验;应用醋酸致小鼠腹腔毛细血管通透性增高、二甲苯致小鼠耳廓肿胀、角叉菜胶致大鼠足跖肿胀及大鼠棉球肉芽肿4种动物模型进行抗炎药效学试验。结果:藁本内酯3个剂量组均能明显延迟小鼠舔后足时间(P<0.05或P<0.01),并能减少醋酸引起的小鼠扭体反应,镇痛率分别为61.21%,48.12%和43.61%;并且藁本内酯各剂量组对醋酸诱发小鼠腹腔毛血管通透性增高的抑制率分别为64.84%,57.85%和49.67%;对二甲苯诱发的小鼠耳廓肿胀有明显的抑制率分别为52.06%,41.51%和34.21%;对角叉菜胶引起的足跖肿胀,在1～3 h的抑制率在42%以上;而对棉球肉芽增生的抑制率达52.64%,50.32%,45.33%。结论:藁本内酯具有一定的镇痛作用,对急、慢性炎症均有明显抑制作用。

Anti-inflammatory and Analgesic Effects of Ligustilide

Objective: To evaluate the anti-inflammatory and analgesic effects of ligustilide. Method: The pharmacodynamic effects were observed in 2 analgesic tests and 4 inflammatory models in experimental animals,including:hot plate test in mice, writhing reaction induced by acetic acid in mice, increased permeability of abdominal cavity induced by acetic acid in mice, auricular edema induced by dimethy benzene in mice, paw swelling caused by carrageenan in rats, cotton-pellet granuloma in rats. Result: Ligustilide (100,60,20 mg ·kg^-1) could delay the latency of licking of the hind paw in hot plate test.And it could reduce the times of writhe response actions,and the inhibition rates were 61.21%,48.12%,43.61% accordingly.Ligustilide could distinctively inhibit the increase in permeability of abdominal cavity induced by acetic acid in mice,and the inhibition rates were 64.84%,57.85%,49.67% respectively.Ligustilide could significantly inhibit the auricular edema induced by dimethy benzene,and the inhibition rates were 52.06%,41.51%,34.21%.Ligustilide could significantly inhibit the paw swelling caused by carrageenan in rats,and the inhibition rates were more than 42% from 1 to 3 hour.Ligustilide could significantly inhibit the cotton-pellet granuloma in rats,and the inhibition rates were 52.64%,50.32%,45.33%. Conclusion: Ligustilide has an analgestic effect and can significant inhibit acute and chronic inflammation in animals.