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小柴胡汤含药血清对肝癌HepG-2细胞的影响
引用本文:李然,刘立萍,马骥,贾立龙,李海波.小柴胡汤含药血清对肝癌HepG-2细胞的影响[J].中国实验方剂学杂志,2013,19(5):217-220.
作者姓名:李然  刘立萍  马骥  贾立龙  李海波
作者单位:1. 辽宁中医药大学基础医学院,沈阳,110847
2. 辽宁中医药大学第一临床学院,沈阳,110847
基金项目:辽宁省科技厅资助项目(20102142);辽宁省教育厅资助项目(L2011165)
摘    要:目的:探讨小柴胡汤含药血清抑制HepG-2肝癌细胞增殖的作用机制.方法:大鼠灌服小柴胡汤3.69 g·kg-1,空白组给予等量蒸馏水,1次/d,连续7d,末次给药1h后腹主动脉取血制备小柴胡汤含药血清.以HepG-2肝癌细胞为研究对象,实验分为10%空白血清组,5%,10%,20%含药血清组.采用倒置显微镜观察含药血清对HepG-2细胞作用后的形态改变,噻唑蓝(MTT)法测定其对生长抑制的影响,流式细胞术分析细胞凋亡的情况,罗丹明123(Rh123)荧光染色观察细胞线粒体膜电位的变化.结果:与空白血清组比较,小柴胡汤含药血清能够抑制HepG-2肝癌细胞增殖,10%含药血清抑制细胞增殖具有时间依赖的特点;流式检测结果表明,10%小柴胡汤含药血清作用HepG-2肝癌细胞8h后,凋亡率从6.78%上升至19.51%;Rh123荧光染色显示,细胞线粒体膜电位明显降低.结论:小柴胡汤含药血清能够抑制HepG-2肝癌细胞增殖,促进其凋亡,线粒体途径参与了该过程.

关 键 词:小柴胡汤  含药血清  肝癌  HepG-2
收稿时间:9/4/2012 12:00:00 AM

Effects of Xiaochaihu Decoction Containing Drug Serum on HepG-2 Cell
LI Ran,LIU Li-ping,MA Ji,JIA Li-long and LI Hai-bo.Effects of Xiaochaihu Decoction Containing Drug Serum on HepG-2 Cell[J].China Journal of Experimental Traditional Medical Formulae,2013,19(5):217-220.
Authors:LI Ran  LIU Li-ping  MA Ji  JIA Li-long and LI Hai-bo
Institution:Basic Medical Sciences,Liaoning University of Traditional Chinese Medicine(TCM),Shenyang 110847,China;Basic Medical Sciences,Liaoning University of Traditional Chinese Medicine(TCM),Shenyang 110847,China;Basic Medical Sciences,Liaoning University of Traditional Chinese Medicine(TCM),Shenyang 110847,China;First Clinical Academy of Liaoning University of TCM,Shenyang 110847,China;Basic Medical Sciences,Liaoning University of Traditional Chinese Medicine(TCM),Shenyang 110847,China
Abstract:Objective:To investigate the effects of Xiaochaihu decoction (XCHD) containing drug serum on the cell proliferation of hepatocarcinoma HepG-2 cell. Method: XCHD containing drug serum was prepared. The cells were separated into four groups: 10% control serum group, 5% XCHD containing drug serum, 10% XCHD containing drug serum and 20% XCHD containing drug serum. The HepG-2 cell morphologic changes were observed using the inverted microscope; HepG-2 cell viability was measured by MTT assay. Apoptosis was detected by flow cytometry assay. Mitochondrial membrane potential changes were measured using fluorescent rhodamine123. Result: Compared with the control group, XCHD containing drug serum could significantly inhibit the proliferation of HepG-2 cell and the inhibition effect of 10% XCHD containing drug serum exhibited time-dependent relationship; flow cytometry results showed that after being treated with 10% XCHD containing drug serum, the apoptosis rate of HepG-2 cell elevated from 6.78% to 19.51%; rhodamine123 staining revealed that the mitochondrial membrane potential was reduced. Conclusion: XCHD containing drug serum could inhibit the proliferation of hepatocarcinoma HepG-2 cells and promote the apoptosis rate. The main mechanism of XCHD inhibiting HepG-2 cell is related with mitochondrial function.
Keywords:Xiaochaihu decoction  containing drug serum  hepatocarcinoma  HepG-2 cell
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