丹蛭降糖胶囊对糖尿病胰岛素抵抗大鼠骨骼肌葡萄糖转运体Ⅳ基因表达的影响

目的：观察丹蛭降糖胶囊对糖尿病胰岛素抵抗模型大鼠骨骼肌葡萄糖转运体Ⅳ（GLUT4）基因表达的影响，以初步明确其作用靶点。方法：雄性Wistar大鼠随机分为空白组、模型组、中药低剂量组、中药高剂量组、西药组、中西药合用组。空白组喂以普通饲料，模型组与各治疗组注射小剂量链脲佐菌素并喂以高热量饲料，常规测定各组大鼠治疗前后的体重、空腹血糖和治疗后各组大鼠的空腹血脂、血清胰岛素水平，计算胰岛素敏感性指数，用Northern印迹和杂交法测定骨骼肌GLUT4基因的表达。结果：丹蛭降糖胶囊能有效地减轻模型大鼠体重，能明显降低大鼠的空腹血糖和胰岛素水平，改善脂代谢紊乱状况，能增加胰岛素抵抗（IR）大鼠骨骼肌中GLUT4 mRNA的表达，从而改善了外周组织的胰岛素敏感性。结论：丹蛭降糖胶囊对2型糖尿病大鼠 IR有显著的改善作用，提示其机制可能与增加IR大鼠骨骼肌中GLUT4 mRNA的表达有关。

The Effect of DZJT-Capsule on the GLUT4 Gene Expression in Skeletal Muscle of Diabetic Rat

Objective:To investigate Danzhi Jiangtang Capsule's effect on the GLUT4 gene expression in skeletal muscle of diabetic IR rat and to find out its functional target.Methods:Male Wistar rats were randomly divided into six groups(normal group,model group,Rosiglitazone Maleate Tablets treating group,Rosiglitazone Maleate Tablets and high dose of Danzhi Jiangtang Capsule treating group,low dose of Danzhi Jiangtang Capsule treating group,high dose of Danzhi Jiangtang Capsule treating group).The rats of normal group were fed with common food while the others with high calorie food and injected with low dose of STZ.The body weigh,fasting blood glucose(FBG),fasting insulin(FIns),blood fat of all rats were measured.Insulin sensitivity index(ISI) was calculated accordingly,and the expression of GLUT4 gene in skeletal muscle of diabetic IR rat was also measured.Results:Danzhi Jiangtang Capsule could reduce body weight,FBG level and FIns level,and improve blood fat condition.Danzhi Jiangtang Capsule could increase the expression of GLUT4 gene in skeletai muscle of diabetic IR rat,thereby it improved insulin sensitivity of peripheral tissue.Conclusions:Danzhi Jiangtang Capsule can ameliorate diabetic IR condition of the model rats and its mechanism is probably related to its effect of enhancing the genic expressional level of GLUT4.