| 桂蚕沙多糖组分的制备与GC-MS分析 |
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| 引用本文： | 李奇豫,张贵君,李素丽,徐蓓蕾,张雅楠,张智圆,董文茜.桂蚕沙多糖组分的制备与GC-MS分析[J].中国实验方剂学杂志,2012,18(8):113-115. |
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| 作者姓名： | 李奇豫 张贵君 李素丽 徐蓓蕾 张雅楠 张智圆 董文茜 |
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| 作者单位： | 1. 北京中医药大学中药学院,北京,100102
2. 广西大学农学院,南宁,530004 |
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| 基金项目： | 广西科技攻关与新产品试制项目(桂科攻10124008-14) |
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| 摘 要： | 目的:制备桂蚕沙的多糖并分析其组分,为专属性鉴定特征的建立奠定基础.方法:桂蚕沙多糖制备流程脱脂后水提醇沉→三氯乙酸除蛋白→大孔吸附树脂纯化→活性炭脱色.桂蚕沙多糖组分的确定采用衍生化后GC-MS分析.结果:桂蚕沙多糖提取率为23.5％.桂蚕沙多糖组分:核糖-木糖-鼠李糖-半乳糖-吡喃葡萄糖-阿拉伯糖(1.97∶3.14∶0.58∶0.92∶5.62).结论:气相色谱-质谱联用技术能够有效的分析桂蚕沙中的多糖组分.
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| 关 键 词： | 桂蚕沙 多糖组分 气相色谱-质谱 专属性鉴定特征 |
| 收稿时间： | 2011/12/10 0:00:00 |
Production of Polysaccharide Components from Faeces Bombycis Gui and Analysis by GC-MS |
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| LI Qi-yu,ZHANG Gui-jun,LI Su-li,XU Bei-lei,ZHANG Ya-nan,ZHANG Zhi-yuan and DONG Wen-qian.Production of Polysaccharide Components from Faeces Bombycis Gui and Analysis by GC-MS[J].China Journal of Experimental Traditional Medical Formulae,2012,18(8):113-115. |
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| Authors: | LI Qi-yu ZHANG Gui-jun LI Su-li XU Bei-lei ZHANG Ya-nan ZHANG Zhi-yuan and DONG Wen-qian |
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| Institution: | School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;Agricultural College of Guangxi University, Nanning 530004, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | Objective: To produce the polysaccharide components from Faeces Bombycis Gui and analyze its composition. Method: Polysaccharide component of Faeces Bombycis Gui was extracted by the methods of water extracting-alcohol precipitating after degreasing, and then removed protein with 10% content of trichloroa cetic acid (TCA), purified with AB-8 macroreticular resin and decolored by active carbon. Finally, the polysaccharide component after derivatization was analyzed by gas chromatography-mass. Result: The yield of crude polysaccharide could attain 23.5%. The polysaccharide of Faeces Bombycis Gui was composed of ribose, xylose, rhamnose, galactose, glucopyranose and arabinose. The proportion of it was 1.97 :3.14 :0.58 :0.92 : 5.62. Conclusion: The GC-MS method could analyze the polysaccharide components of Faeces Bombycis Gui effectively. |
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| Keywords: | Faeces Bombycis Gui polysaccharide components GC-MS identification specificity |
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