HPLC同时测定忍冬与红腺忍冬中3种成分的含量

目的:建立同时测定忍冬与红腺忍冬中绿原酸、芦丁和槲皮素的HPLC分析方法.方法:依利特Hypersil ODS (4.6 mm×250 mm,5 μm),流动相乙腈-0.05％磷酸梯度洗脱,检测波长327 nm,柱温25℃,进样10μL,流速1.0 mL· min-1.结果:线性范围内3种成分的标准曲线呈良好的线性关系,加样回收率在97％～101％,精密度和重复性的RSD均符合定量分析要求.结论:该方法准确、简便,具有良好的重复性和稳定性,可用于忍冬及红腺忍冬药材的质量控制.

Simultaneously Determine the Contents of Three Components in Honeysuckle and the Red Gland Honeysuckle by HPLC

Objective:HPLC method was establisheet for simultaneous determinaton of chlorogenic acid,rutin and quercetin in honeysuckle and red gland honeysuckle.Method: The separation was performed on a Elite Hypersil ODS C18 column(4.6 mm×250 mm,5 μm).acetonitril-0.05% H3PO4 was used as the mobile phase.The detection wavelength was at 327 nm.The temperature was maintained at 25 ℃.The volume was 10 μL.The flow rate was 1.0 mL·min-1.Result: Within Linear range,the three components of the standard curve are in good linear relationship within linear range.The average recovery was 97%-101% recovery.RSD of precision and repeatability accord with quantitative analysis requirements.Conclusion: This method is accurate,simple,with good repeatability and stability and can be used for honeysuckle and red gland honeysuckle quality control.