| HPLC测定葛根饮片中5个成分的含量 |
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| 引用本文： | 章军,薛翠娟,荆文光,王跃生,刘安.HPLC测定葛根饮片中5个成分的含量[J].中国实验方剂学杂志,2012,18(7):108-111. |
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| 作者姓名： | 章军 薛翠娟 荆文光 王跃生 刘安 |
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| 作者单位： | 1. 中国中医科学院中药研究所,北京,100700
2. 江西中医学院,南昌,330006 |
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| 基金项目： | 中药质量控制技术国家工程实验室课题(2010DZHT001) |
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| 摘 要： | 目的:建立HPLC测定葛根饮片中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷元-8-C-芹菜糖-(1→6)葡萄糖苷和大豆苷5个成分含量的方法.方法:采用Zorbax C18色谱柱(4.6 mm× 250 mm,5 μm),以甲醇-0.02％磷酸(25∶75)为流动相,检测波长250 nm.结果:方法学考察合格,并测定了12批葛根饮片中5个成分的含量.结论:该方法简便、快速、准确度高,可作为葛根饮片的质量标准.
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| 关 键 词： | 葛根 3’-羟基葛根素 葛根素 3’-甲氧基葛根素 大豆苷元-8-C-芹菜糖-(1→6)葡萄糖苷 大豆苷 |
| 收稿时间： | 2011/9/15 0:00:00 |
Determination of 5 Active Components in Radix Pueraria lobata by HPLC |
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| ZHANG Jun,XUE Cui-juan,JING Wen-guang,WANG Yue-sheng and LIU An.Determination of 5 Active Components in Radix Pueraria lobata by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(7):108-111. |
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| Authors: | ZHANG Jun XUE Cui-juan JING Wen-guang WANG Yue-sheng and LIU An |
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| Institution: | Institute of Chinaese Materia Medica, China Academy of chinese Medical Science, Beijing 100700, China;Jiangxi University of Traditional Chinaese Medicine, Nanchang 330006, China;Institute of Chinaese Materia Medica, China Academy of chinese Medical Science, Beijing 100700, China;Institute of Chinaese Materia Medica, China Academy of chinese Medical Science, Beijing 100700, China;Institute of Chinaese Materia Medica, China Academy of chinese Medical Science, Beijing 100700, China |
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| Abstract: | Objective:To establish a HPLC method for determination of 3′-hydroxy puerarin,puerarin,3′-methoxy puerarin,daidzein-8-c-apiosyl-(1→6) glucoside and daidzin in radix Pueraria lobata.Method: A Zorbax C18(4.6 mm×250 mm,5 μm) was used;the mobile phase was methanol-0.02% phosphoric acid(25∶75).The detection wavelength was at 250 nm;the flow rate was 1.0 mL·min-1 and the column temperature was maintained at 40 ℃.Result: The accuracy,precision,sensitivity,specificity and linearity of this method met the requirements.The method was applied to 12 batches of radix Pueraria lobata.Conclusion: The method is rapid,simple and accurate,which can be used in the quality control in radix Pueraria lobata. |
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| Keywords: | radix Pueraria lobata 3′-hydroxy puerarin puerarin 3′-methoxy puerarin daidzein-8-c-apiosyl-(1→6) glucoside daidzin |
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