三七多糖的含量测定方法及不同部位多糖的含量变化研究

目的:建立三七多糖的检测方法,分析测定三七茎叶、筋条、花和剪口的多糖含量,为三七的质量评价提供依据.方法:用80％乙醇提取还原性糖,然后用水提取三七多糖,采用蒽酮-硫酸比色法于528 nm波长处用分光光度法测定多糖的含量.结果:三七不同部位中多糖含量有明显差异,三七筋条中多糖含量最高,三七茎叶中多糖含量最低.结论:所用含量检测方法简便、快速、准确,可用于不同部位三七多糖的质量检测和控制,三七筋条、花、茎叶和剪口都有一定的多糖开发价值.

Studies on Content Determinationthe Polysaccharides in Panax notoginseng and Change of Polysaccharides in Panax notoginseng Collected at Different Parts

Objective:Detection of polysaccharide established on Panax notoginseng, determination of polysaccharide content in stem and leaf, ribs, flowers and rhizoma to provide the basis for quality evaluation on P. notoginseng. Method:The content of polysaccharides was determined by anthrone sulfuric acid method and detected at 528 nm by Shimadzu uv-2450. Result:There were significant defferences of the content of the extracted polysaccharides among P. notoginseng from different parts. Conclusion:Used method for the assay is simple, rapid, accurate, and can be used in quality inspection and control in different parts of P. notoginseng polysaccharide; Stem and leaf, ribs, flowers and rhizoma have some development value of polysaccharide.