新疆阿魏树脂不同分离部位对结肠癌细胞HCT116的抑制作用

目的: 分析新疆阿魏树脂不同分离部位对结肠癌细胞HCT116抑制作用的活性，并确定其有效活性部位。 方法: 通过磺酰罗丹明B比色法（SRB）与流式细胞术以细胞密度1×10⁵个mL，药物质量浓度250，125，62.5，31.25，15.6 mg·L^-1（流式细胞术药物浓度为62.5 mg·L^-1），检测新疆阿魏树脂不同分离部位（石油醚部位、乙酸乙酯部位，甲醇部位）对结肠癌细胞 HCT116药物作用24 h后的增殖抑制与凋亡作用，以IC₅₀和总凋亡率作为指标衡量其各分离部位抑制肿瘤的活性效能。 结果: 石油醚部位:对结肠癌细胞HCT116增殖抑制IC₅₀ 68.7 mg·L^-1，总凋亡率为（43.4±1.1）%;乙酸乙酯部位:IC₅₀43.7 mg·L^-1， 总凋亡率为（56.2±0.9）%;甲醇部位:IC₅₀59.6 mg·L^-1，总凋亡率为（46.7±3.1）%。 结论: 新疆阿魏树脂乙醇提取物的乙酸乙酯萃取部位对结肠癌细胞HCT116细胞毒性作用较强并能促进其大量凋亡，初步确定为新疆阿魏树脂抗肿瘤活性部位。

Inhibition Activity of Different Separation Parts of Ferula sinkiangensis Resin on HCT116 Human Colon Cancer Cell

Objective: To study inhibitory activity the different separation parts of Ferula sinkiangensis resin on HCT116 human colon cancer cell and determine its effective active site. Method: The effects of the different separate parts of F. sinkiangensis resin (petroleum ether parts,ethyl acetate parts,methanol parts) on colon cancer cells HCT116 proliferation and apoptosis were observed, IC₅₀ and total apoptosis rate were used toevaluate tumor suppression activity performance by SRB and flow cytometry with cell concentration 1×10⁵/mL, and drug concentration of 250,125,62.5,31.25,15.6 mg· L^-1 (flow cytometry drug concentration was 62.5 mg· L^-1). Result: IC₅₀ of petroleum ether unit was 68.7 mg· L^-1,the total apoptosis rate was (43.4±1.1)%;IC₅₀ of ethyl parts:43.7 mg· L^-1,total apoptosis rate was (56.2±0.9)%;IC₅₀methanol Department of 59.6 mg· L^-1,total apoptosis rate was (46.7±3.1)%. Conclusion: Ferula resin ethanol extract ethyl acetate fraction can promote their apoptosis,initially identified as anti-tumor activity in F.sinkiangensis resin parts.