淫羊藿-女贞子对GIOP大鼠骨密度和激素水平的相关性分析

目的: 研究糖皮质激素性骨质疏松（GIOP）大鼠内源性激素水平与骨密度的相关性，探讨淫羊藿-女贞子配伍抗GIOP的作用机制。 方法: SD大鼠60只，雌雄各半，随机分为正常对照组、模型对照组、女贞子组、淫羊藿组、淫羊藿-女贞子组、钙尔奇组。地塞米松1 mg·kg^-1，im，2次／周，建立骨质疏松模型。造模同时各给药组灌服相应药物，女贞子组灌服女贞子水煎液9.5 g·kg^-1，淫羊藿组灌服淫羊藿水煎液9.5 g·kg^-1，淫羊藿-女贞子组灌服淫羊藿-女贞子（4∶3）水煎液9.5 g·kg^-1，钙尔奇组灌服钙尔奇碳酸钙D3片0.277 3 g·kg^-1。8周后处死取材，放射免疫法检测血清皮质醇（COR）、睾酮（T）、雌二醇（E₂）含量;双能X射线骨密度仪测量离体股骨骨密度（BMD）;并对实验数据进行相关性分析。 结果: 模型对照组大鼠血清COR，T，E₂含量以及股骨不同部位BMD均显著降低（P<0.05或0.01）;与模型对照组比较，各给药组均可显著上调血清COR水平（P<0.05或0.01），淫羊藿-女贞子组血清T含量显著升高（P<0.05），女贞子组、淫羊藿-女贞子组血清E₂含量显著升高（P<0.05或0.01），且各用药组不同部位BMD均较模型对照组显著升高（P<0.05或0.01）;相关性分析结果显示BMD与血清COR，T，E₂水平呈显著正相关（P<0.05或0.01）。 结论: GIOP大鼠BMD与内源性激素水平密切相关，淫羊藿-女贞子配伍对激素水平的影响可能是其防治GIOP的机制之一。

Analysis of Correlation of Combination of Epimedii Folium and Ligustri Lucidi Fructus on Bone Mineral Density and Hormone Levels in Glucocorticoid-induced Osteoporosis Rats

Objective: To explore the correlations between bone mineral density(BMD) and endogenous hormone levels,and then investigate the mechanism of anti-GIOP of combination of Epimedii Folium and Ligustri Lucidi Fructus. Method: Sixty SD rats were randomly divided into 6 groups:normal control group,model group,Epimedii Folium group,Ligustri Lucidi Fructus group,Epimedii Folium and Ligustri Lucidi Fructus mixture group(compatibity group) and positive drug group. All rats received intramuscular injection of dexamethasone (1 mg· kg^-1,twice a week) for 8 weeks to establish osteoporosis model except those in the control group,rats in treatment groups were treated with intragastric administration of traditional Chinese herbs or positive drug respectively,Ligustri Lucidi Fructus group was given Ligustrum lucidum with 9.5 g· kg^-1,Epimedii Folium group was given Epimedium with 9.5 g· kg^-1, the compatibility group were given Epimedii Folium-Ligust rilucidi Fructus with 9.5 g· kg^-1 the positive group was administered Caltrate calcium D3 tablets with 0.277 3 g·kg^-1. Eight weeks later, serum corticosteroid (COR),testosterone (T) and estradiol (E₂) were determined by radioimmunoassay,and BMD of femur was measured by dual-energy X-ray absorptiometry. Result: The content of serum COR,T,E₂ and BMD of modle group were lower than normal control group(P<0.05 or P<0.01). Compared with model group,serum levels of COR were upregulated significantly in each administration group(P<0.05 or P<0.01),serum T levels in compatibility group and E₂ levels in Ligustri Lucidi Fructus group and compatibility group were increased significantly(P<0.05 or P<0.01),BMD increased obviously in each treatment group(P<0.05 or P<0.01). The correlation analysis showed that BMD was positively correlated with serum hormone levels(P<0.05 or P<0.01). Conclusion: BMD was closely related to endogenous hormone levels in glucocorticoid-induced osteoporosis rats,which indicate that the effect of combination of Epimedii Folium and Ligustri Lucidi Fructus on hormone levels may be one of the mechanisms of its prevention and treatment of GIOP.