黄芪黄酮与葛根黄酮配伍对肝脏糖脂代谢的影响

目的:明确黄芪黄酮与葛根黄酮配伍对糖尿病大鼠糖脂代谢的影响。方法:SPF级SD雄性大鼠66只,按血糖随机分为6组,11只/组。除正常组外,其他各组均按46 mg·kg-1予以1次性ip链脲佐菌素(STZ)诱导糖尿病模型。造模同日ig给药,黄芪黄酮组、葛根黄酮组、黄芪黄酮+葛根黄酮组分别给予0.039,0.036,0.075 g·kg-1;阳性药组给予金芪降糖片1.47 g·kg-1;正常组、模型组ig给予等量蒸馏水10 m L·kg-1。各组连续给药30 d。分别检测血糖、血清胆固醇(cholesterol,CHO)与甘油三酯(triglyceride,TG)含量,检测肝脏胰岛素(insulin,INS)、葡萄糖转运体4(glucose transporter 4,GLUT4)、脂联素(adiponectin,ADPN)及瘦素(leptin,LEP)浓度。运用单因素方差分析法分析两种药物的单独效应,析因设计方差分析法分析两种药物合用的交互效应。结果:与正常组比较,模型组大鼠血糖、血清CHO,TG均升高(P0.05),肝组织INS,GLUT4,ADPN及LEP浓度均下降(P0.05)。与模型组比较,黄芪黄酮组糖尿病大鼠血糖水平明显降低(P0.05),葛根黄酮组变化不明显,二药合用未呈现协同作用;黄芪黄酮组、葛根黄酮组CHO,TG含量均明显降低,但合用效果不如单味药;黄芪黄酮、葛根黄酮均能明显升高肝脏GLUT4水平(P0.05);二药对肝脏INS,GLUT4及LEP的影响,合用比单味药效果好,二药存在协同作用。结论:黄芪黄酮与葛根黄酮配伍能够协同调节INS,GLUT4及LEP水平而实现改善糖尿病糖脂代谢紊乱的作用。

Effect of Compatibility of Astragalus Flavonoids and Kudzu Flavonoids on Glucose and Lipid Metabolism in Liver Tissue

Objective: To explore the effect of compatibility of astragalus flavonoids and kudzu flavonoids on glucose and lipid metabolism in diabetes mellitus(DM)rats. Method: Sixty-six SD male rats were randomly divided into six groups according to blood glucose, which was normal group, model group, positive control group, astragalus flavonoids group, kudzu flavonoids group, and astragalus flavonoids and kudzu flavonoids group,11 rats in each group. In addition to the normal group, the other groups were induced by streptozotocin (STZ, 46 mg · kg^-1) to make the DM model. At the same time, the drugs were ig given. Astragalus flavonoid group, kudzu flavonoid group, astragalus flavonoid and pueraria flavonoid group were respectively given at dose of 0.039, 0.036, 0.075 g · kg^-1;positive control group was given Jinqi Jiangtang tablets at dose of 1.47 g · kg^-1;normal group and model group were given the same amount of distilled water(10 mL · kg^-1). After administration for 30 days, the levels of blood glucose, serum cholesterol (CHO) and triglyceride (TG) were tested, and hepatic insulin (INS), glucose transporter 4 (GLUT4), adiponectin (ADPN), and leptin (LEP) were tested as well. One-way ANOVA was used to analyze the single effect of astragalus flavonoids and kudzu flavonoids on glucose and lipid metabolism, and factorial design analysis of variance was used to analyze their interactions as well. Result: In DM rats, the contents of blood glucose, CHO and TG were statistically increase (P<0.05), and the concentrations of hepatic INS, GLUT4, ADPN and LEP were statistically decreased (P<0.05). Astragalus flavonoids could reduce blood glucose level in rats effectively (P<0.05), while the effect of kudzu flavonoids was not obvious, and there was no synergistic effect between the two drugs. Both astragalus flavonoids and kudzu flavonoids could reduce serum TG and CHO, but the combined effect was not obvious compared to any single drug, and there were antagonistic effects between the two drugs. Both astragalus flavonoids and kudzu flavonoids could raise the level of hepatic GLUT4(P<0.05).In terms of hepatic INS, GLUT4 and LEP levels, the combined effects were better than any single drug, it showed that there were synergistic effects between the two drugs on them. Conclusion: Compatibility of astragalus flavonoids and kudzu flavonoids could improve the glucose and lipid metabolism by regulating INS, GLUT4 and LEP levels synergistically.