虎杖药材UPLC特征图谱研究

目的:建立虎杖药材的超高效液相色谱(UPLC)特征图谱的质量评价方法.方法:采用Waters Acquity UPLCBEH C18色谱柱(2.1 mm×100 mm,1.7 μm),流动相乙腈-0.5％乙酸水溶液,梯度洗脱,流速0.3 mL·min-1,柱温为35℃,检测波长290 nm.采用聚类分析,主成分分析,特征图谱相似度分析3种方法对38批不同产地的虎杖药材UPLC特征图谱进行评价.结果:建立了虎杖药材的UPLC特征指纹图谱共有模式,共标定了11个共有峰,指认其中4个共有峰为虎杖苷、白藜芦醇、大黄素和大黄素甲醚.36批虎杖药材相似度＞0.9；2批药材相似度为0.733,0.896.聚类分析和主成分分析结果表明可将药材分为3类,不同类别间质量有差异.结论:本研究建立的虎杖药材的特征图谱特征性和专属性强,且方法快速、简便、可靠,可用于全面控制市场流通虎杖药材的质量.

Studies on the UPLC Fingerprint and Quality Evaluation of Crude Drug of Polygonum cuspidatum

Objective:A method of UPLC fingerprinting analysis was developed for the quality evaluation of crude drug of Polygonum cuspidatum.Method: The Waters Acquity UPLC BEH C18(2.1 mm×100 mm,1.7 μm) column was used.Acetonitrile and water containing 0.5% acetic acid were used as mobile phase of UPLC with gradient elution,monitored with the wavelength of 290 nm.Flow rate was set at 0.3 mL·min-1 and column temperature was 35 ℃.Thirty eight batches of P.cuspidatum samples collected from different areas and markets were analyzed for similarity analysis(SA),hierarchical clustering analysis(HCA) and principle component analysis(PCA).Result: The chromatographic fingerprint was completed with eleven recognizable peaks and four of them(polydatin,resveratrol,emodin,physcion) determined.Thirty eight batches of samples were classified as three clusters by HCA and PCA,and thirty six of them exhibited similarity more than 0.900 by similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(2004B Version).Conclusion: The established method could rapidly attain the identification and quality evaluation of crude drug of P.cuspidatum.