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拆方研究降香总萜、降香黄酮、丹参酮在冠心丹参 方中抗大鼠心肌细胞缺氧/复氧损伤作用
引用本文:黄璜,何欢,王丹辉,王金钱,黄方威,何秀菊,刘丹,范玫玫,何明.拆方研究降香总萜、降香黄酮、丹参酮在冠心丹参 方中抗大鼠心肌细胞缺氧/复氧损伤作用[J].中国实验方剂学杂志,2012,18(2):191-194.
作者姓名:黄璜  何欢  王丹辉  王金钱  黄方威  何秀菊  刘丹  范玫玫  何明
作者单位:1. 南昌大学药学院药理学与分子治疗学教研室,南昌,330006
2. 中药固体制剂制造技术国家工程研究中心,南昌,330006
基金项目:科技部国际科技合作项目(2006DFB33080)
摘    要:目的:研究冠心丹参处方中,分别除去降香总萜和降香总黄酮,或是加入丹参酮,研究其对抗心肌细胞缺氧/复氧(A/R)损伤的保护作用,探讨各组分对药效的贡献.方法:取培养4d的大鼠乳鼠心肌细胞,随机分为:对照组(Cont组)、(1)A/R组、(2)1%的DSMO对照组、(3)冠心丹参组、(4)~(6)降香总萜与/和降香总黄酮阴性组、或是冠心丹参处方中分别加入终浓度为1,2,4 mg的丹参酮;检测细胞存活率、超氧化物歧化酶(SOD)、过氧化氢酶(catalase)、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量及孵育液乳酸脱氢酶(LDH)活性.结果:在冠心丹参处方中分别除去降香总萜与/和降香总黄酮后,样品对抗心肌细胞的A/R的作用显著减弱,尤其以反映细胞抗脂质氧化状态指标SOD,catalase,GPx活性以及MDA含量更为明显;而加人丹参酮后,其对抗心肌细胞A/R的作用有不同程度的增强,尤其以反映细胞存活状态指标细胞存活率以及LDH更为明显,以2 mg丹参酮最为理想.结论:冠心丹参处方中在对抗心肌细胞A/R损伤的作用上,降香总萜和降香总黄酮不可缺如;加入2 mg丹参酮可增强其心肌保护作用.

关 键 词:冠心丹参胶囊  心肌细胞  缺氧/复氧损伤  降香总萜  降香总黄酮  丹参酮
收稿时间:2011/3/29 0:00:00

Separate Experimental Study of Effect of Lignum Acronylchiae Total Flavonoids, Lignum Acronylchiae Oil, Tanshinone in the Guanxin Danshen Capsule against Cardiomyocyts Anoxia/Reoxygenation Injury
HUANG Huang,HE Huan,WANG Dan-hui,WANG Jin-qian,HUANG Fang-wei,HE Xiu-ju,LIU Dan,FAN Mei-mei and HE Ming.Separate Experimental Study of Effect of Lignum Acronylchiae Total Flavonoids, Lignum Acronylchiae Oil, Tanshinone in the Guanxin Danshen Capsule against Cardiomyocyts Anoxia/Reoxygenation Injury[J].China Journal of Experimental Traditional Medical Formulae,2012,18(2):191-194.
Authors:HUANG Huang  HE Huan  WANG Dan-hui  WANG Jin-qian  HUANG Fang-wei  HE Xiu-ju  LIU Dan  FAN Mei-mei and HE Ming
Institution:Department of Pharmacology & Molecular Therapeutics, Nanchang University School of Pharmaceutical Science, Nanchang 330006, China;Department of Pharmacology & Molecular Therapeutics, Nanchang University School of Pharmaceutical Science, Nanchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China;Department of Pharmacology & Molecular Therapeutics, Nanchang University School of Pharmaceutical Science, Nanchang 330006, China;National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China;Department of Pharmacology & Molecular Therapeutics, Nanchang University School of Pharmaceutical Science, Nanchang 330006, China
Abstract:Objective : To investigate the protective effect on cardiomyocyts against anoxia/reoxygenation(A/R) injury in Guanxin Danshen compounding of the best ratio, if lignum acronylchiae oil and/or lignum acronylchiae total flavonoids was removed, or tanshinone from salvia miltiorrhiza was added, of the contribution of various components on the efficacy. Method : Taken cultured neonatal rat ventricular cardiomyocytes for 4 days were randomly divided into seven groups: Control group; (1) A/R group; (2) 1% DSMO group; (3) the group treated with the best ratio; (4)-(6): these groups respectively treated with lignum acronylchiae oil and/or lignum negative total flavonoids, or salviol of different dose 1, 2, 4 mg added; cell viability, superoxide dismutase(SOD), Catalase, glutathione peroxidase(GSH-Px) activities, malondialdehyde(MDA) contents and lactate dehydrogenase(LDH) activitied were detected. Result : In Guanxin Danshen compounding of the best ratio, the protective effect of cardiomyocyte against A/R injury was significantly decreaed if lignum acronylchiae oil and/or lignum acronylchiae total flavonoids was removed, these changes of anti-lipid oxidation indexes such as SOD, Catalase, GSH-Px activities and MDA contents were more obvious than other indexes; Once salviol was added, the protective effect on cardiomyocyte against A/R was enchanced with different degree, espcially LDH and cell viability was obvious and 2 mg salviol was the best protective effect. Conclusion : In the Guanxin Danshen compounding of the best ratio, lignum acronylchiae oil and lignum acronylchiae total flavonoids were necessary; Adding 2 mg salviol could enchance the protective effect on cardiomyocyte.
Keywords:Guanxin Danshen capsule  cardiomyocyte  anoxia-reoxygenation injury  lignum acronylchiae oil  lignum acronylchiae total flavonoids  tanshinone
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