续断通络胶囊质量标准研究

目的:建立续断通络胶囊质量标准.方法:采用薄层色谱法对处方中的狗脊、续断、白芍、槲寄生进行了定性鉴别；采用HPLC,以川续断皂苷Ⅵ为评价指标,色谱柱Agilent C18(4.6 mm×250 mm,5μm),流动相乙腈-水(30∶ 70),检测波长212 nm,流速1.0 mL· min -进行含量测定.结果:供试品色谱中,在与对照品或对照药材色谱相应位置上显相同颜色的斑点,阴性无干扰.川续断皂苷Ⅵ在0.45～3.6μg线性关系良好(r=0.999 9),平均加样回收率为98.31％,RSD 1.42％.结论:方法简便、专属性强、重复性好,可有效的控制续断通络胶囊的质量.

Studies on Quality Control of Xuanduan Tongluo Capsule

Objective:To establish a method for quality control of Xuanduan Tongluo capsule.Method:To establish qualitative analysis in teasel,rhizoma cibotii,radix paeoniae rubra and parasitic loranthus.To determine the content of teasel saponin Ⅵ by HPLC,chromatographic column was Agilent-C18 with acetonitrile-water(30∶70)as the mobile phase.The detection wavelength was set at 212 nm and flow rate was 1.0 mL·min-1.Result:Provide to try article color spectrum amid,correspond the spot in same color of location with the collate article or the collate medicine material color spectrum,feminine gender have no jam.Teasel saponin Ⅵ was separated with a good linearity in the sample size ranges of 0.45-3.6 μg,the average recoveries was 98.31%,with RSD 1.42%.Conclusion:The method is simple,specific and reproducible to control the quality of Xuanduan Tongluo Capsule.