石菖蒲水提物对获得性无助模型的抗抑郁作用

目的:探讨石菖蒲水提物(WERAT)在获得性无助模型上的抗抑郁作用及其对神经系统的作用机制.方法:在获得性无助实验中,将大鼠随机分为空白对照组、模型组、丙咪嗪(100 mg·kg-1)组、WERAT(100,200 mg·kg-1)组,连续ig给药5d.利用大鼠穿梭计算机图像实时检测分析系统给予大鼠不可逃避的足底电击建立获得性无助模型,进行条件性回避反应训练,测定大鼠的逃避失败次数;在小鼠5-羟色胺酸(5-HTP)增强实验中,小鼠随机分为空白对照组、氟西汀(30 mg·kg-1)组和WERAT(50,100,200 mg·kg-1)组测定5-HTP诱导的小鼠甩头次数.结果:在获得性无助实验中,模型组动物在第3～5天的逃避失败次数比正常组动物显著增加(分别为P<0.05,P＜0.01,P＜0.01),WERAT 100 mg·kg-1 ·d-1在第3～5天能够使逃避失败次数显著减少(分别为P<0.05,P＜0.01,P＜0.05),WERAT 200 mg· kg -·d-在第4天和第5天更加明显的减少逃避失败次数(P＜0.01).在小鼠5-HTP增强实验中,WERAT 200 mg· kg -显著增加5-HTP诱导的小鼠甩头次数(P＜0.01).结论:WERAT在大鼠获得性无助模型上具有抗抑郁作用,其抗抑郁活性可能与增强5-羟色胺(5-HT)神经系统的功能有关.

Antidepressant Effect of the Water Extract of Acori Tatarinowii Rhizoma in the Learned Helplessness Rat Mode

Objective: To investigate the antidepressant-like effect of water extract of Acori Tatarinowii Rhizoma (WERAT)and its mechanism on the nervous system. Method: In the learned helplessness test, rats were divided randomly into five group: control group, non helpless group, imipramine(100 mg·kg^-1) and WERAT (100,200 mg·kg^-1). The test was administered via the oral rout, and using computer-aided controlling image analysis system for shuttle box, the rats were exprosured to inescapable footshock(IS) for the learned helplessness model. Then the conditioned avoidance testing was processed and the times of escape failure(EF) were recorded. In addition, mice were divided randomly into five groups: control group, fluoxetine (30 mg·kg^-1) and WERAT(50,100,200 mg·kg^-1). 5-hydroxy-DL-tryptophan(5-HTP) induced the head-twitch number of mice were investigated by the accentuation of 5-HTP test. Result: In the learned helplessness test, compared with the control group, the times of EF of IS rats were significantly increased from days 3-5(P<0.05, P<0.01, P<0.01), WERAT at dose of 100 mg·kg^-1 per day significantly reversed the effect of IS on EF from days 3-5(P<0.05,P<0.01, P<0.05), and WERAT at dose of 200 mg·kg^-1 per day also more significantly reversed the effect of IS on EF from days 3-4(P<0.01);WERAT could distinctly increased the frequency of head-twitch in the 5-HTP induced head-twitches test. Conclusions: The results of this study demonstrate that WERAT possesses significant antidepressant-like effect in the learned helplessness mode and its mechanism may be related to enhance the function of 5-hydroxytryptamine nerve systems.