不同果形栀子指纹图谱

目的:比较不同果形栀子药材的HPLC和IR指纹图谱,分析其差异。方法:将48批不同果形栀子药材进行产地加工,建立HPLC及IR指纹图谱,并采用相似度评价,主成分及聚类分析对福建、河南、江西六、七棱栀子进行分析。结果:HPLC指纹图谱,标定了22个共有峰,48批样品中六棱栀子相似度在0.789~0.896,七棱栀子的相似度在0.598~0.103,六、七棱栀子果实相似性较差,所含成分差异较大;运用主成分及聚类分析,将六,七棱栀子分为两大类,福建六棱与河南六棱栀子归为一类,河南七棱与江西七棱栀子归为一类;不同产地相同果形归为一类。IR指纹图谱运用聚类分析,当类间距离为1.35时,栀子药材被分为两大类,六棱栀子归为一类,七棱归为一类;当类间距离为0.875时,福建六棱与河南六棱栀子归为一类;当类间距离为0.756时,河南七棱与江西七棱栀子归为一类。结论:该研究表明不同果形栀子所含化学成分差异较大,采用中药指纹图谱分析,能全面综合评价不同果形栀子药材,为不同果形栀子药材在中医药临床应用中提供参考。

Fingerprints of Gardeniae Fructus with Different Fruit Shapes

Objective:To compare the high performance liquid chromatography (HPLC) and infrared spectroscopy (IR) fingerprints of different fruit-shaped Gardenia jasminoides and analyze their differences. Method:The 48 batches of Gardeniae Fructus samples were processed to establish HPLC and IR fingerprints,and then the similarity evaluation and principal components cluster analysis were performed on Fujian,Henan,and Jiangxi six and seven edges Gardeniae Fructus samples. Result:HPLC fingerprints:22 common peaks were identified. The similarity was ranged from 0.789 to 0.896 among 6 edges Gardeniae Fructus in 48 samples,and 0.598 to 0.103 among seven edges Gardeniae Fructus. The similarity was low between six and seven edges Gardeniae Fructus in fruit shape, with great difference in components. In the principal components and cluster analysis,the six and seven edges Gardeniae Fructus were divided into two categories:Fujian six edges and Henan six edges Gardeniae Fructus were classified into one same category; Henan seven edges Gardeniae Fructus and Jiangxi seven edges Gardeniae Fructus were classified into one same category; the same fruit shape in different places was classified into one category. Infrared fingerprinting:In the cluster analysis,when the between-class distance was 1.35,Gardeniae Fructus samples were divided into 2 categories,six edges Gardeniae Fructus and seven edges Gardeniae Fructus; when the between-class distance was 0.875,Fujian 6edges Gardeniae Fructus and Henan six edges Gardeniae Fructus were classified into one category;when the between-class distance was 0.756,Henan seven edges Gardeniae Fructus and Jiangxi seven edges Gardeniae Fructus were classified into one category. Conclusion:This study shows that the contained components are obviously different in different fruit shaped Gardeniae Fructus,and the fingerprinting analysis can be used to evaluate different fruit shaped Gardeniae Fructus materials, providing reference for the clinical application of different fruit shaped Gardeniae Fructus in traditional Chinese medicine.