2种黄檀属植物粗提物的抑菌抗炎作用分析

目的:研究斜叶黄檀和两粤黄檀2种植物粗提物的抑菌和抗炎作用。方法:通过二倍稀释法,考察2种植物粗提物对表皮葡萄球菌、痤疮丙酸杆菌和金黄色葡萄球菌的抑菌作用;将小鼠随机分为空白组、阳性组、斜叶黄檀和两粤黄檀低、中、高剂量组(0.85,1.7,2.6 g·kg~(-1)),共8组,每组8只,采用二甲苯致耳肿胀、鸡蛋清致足肿胀和小鼠痤疮模型研究2种黄檀属植物粗提物的抗炎作用。结果:2种黄檀属植物对表皮葡萄球菌、痤疮丙酸杆菌和金黄色葡萄球菌均有抑菌作用,两粤黄檀的最小抑菌浓度(minimum inhibitory concentration,MIC)分别为5,10,40 g·L~(-1),斜叶黄檀的MIC分别为2.5,2.5,20 g·L~(-1)。2种黄檀属植物的中、高剂量组均能显著减轻二甲苯所致的小鼠耳肿胀度(P0.05,P0.01);斜叶黄檀高剂量组和两粤黄檀中、高剂量组可明显抑制由鸡蛋清致炎3,4,6 h后的足肿胀度(P0.01);斜叶黄檀中剂量组和两粤黄檀中、高剂量组可明显减轻混合菌液引起的耳肿胀度(P0.01)。结论:斜叶黄檀和两粤黄檀均具有显著的抑菌和抗炎作用。

Antibacterial and Anti-inflammatory Effect of Crude Extract of Two Dalbergia Plants

Objective: To study the antibacterial and anti-inflammatory effects of crude extract of Dalbergia pinnata and D. benthamii,two kinds of Dalbergia plants. Method: Two fold dilution method was used to investigate the antimicrobial activity of crude extracts from two plants against Staphylococcus epidermidis, Propionibacterium acnes and S. aureus. Mice were divided into 8 groups randomly, including blank group, positive group, D. benthamii and D. pinnata low dose group, middle dose group and high dose group (0.85, 1.7, 2.6 g·kg^-1), 8 rats in each group. Ear swelling induced by dimethylbenzene, paw swelling induced by egg white and acne model in mice were used to investigate the anti-inflammatory effect of crude extract of two Dalbergia plants. Result: Two Dalbergia plants had antibacterial effects against S. epidermidis, P. acnes, and S. aureus, with minimum inhibitory concentration (MIC) of 5, 10, 40 g·L^-1 for D. benthamii were and 2.5, 2.5, 20 g·L^-1 for D. pinnata respectively. The middle and high dose of two Dalbergia plants could significantly relieve ear-swelling caused by dimethylbenzene in mice (P<0.05,P<0.01). The high dose of D. pinnata and the middle and high dose of D. benthamii could significantly inhibit paw swelling induced by egg white within 3, 4, 6 hours (P<0.01). The middle dose of D. pinnata and the high and middle dose of D. benthamii could significantly alleviate ear swelling induced by mixed bacteria solution (P<0.01). Conclusion: Both D. pinnata and D. benthamii have significant antibacterial and anti-inflammatory effects.